DNA pol ε A Antibody (3C5.1): sc-12728

DNA pol ε A Antibody (3C5.1) is a mouse monoclonal IgG2a kappa light chain antibody that detects DNA pol ε A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-DNA pol ε A antibody (3C5.1) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. DNA polymerase epsilon (DNA pol ε) plays a crucial role in DNA replication, particularly in synthesizing the leading strand, which is essential for maintaining genome integrity. This enzyme is a multi-subunit complex, with its catalytic subunit encoded by the POL2 gene, and is vital for accurate and efficient DNA replication. Proper functioning of DNA pol ε is critical, as replication errors can lead to mutations and genomic instability, which are hallmarks of many cancers. DNA pol ε A monoclonal antibody (3C5.1) helps researchers study interactions with other proteins involved in replication, including the sliding clamp protein PCNA, which enhances activity and processivity. Understanding DNA pol ε dynamics and interactions is important for elucidating DNA replication and repair mechanisms, making DNA pol ε A (3C5.1) antibody an invaluable research tool for studying these fundamental biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DNA pol ε A Antibody (3C5.1) References:

1. Direct interaction of proliferating cell nuclear antigen with the p125 catalytic subunit of mammalian DNA polymerase delta.  |  Zhang, P., et al. 1999. J Biol Chem. 274: 26647-53. PMID: 10480866
2. A third essential DNA polymerase in S. cerevisiae.  |  Morrison, A., et al. 1990. Cell. 62: 1143-51. PMID: 2169349
3. Discontinuous DNA synthesis by purified mammalian proteins.  |  Goulian, M., et al. 1990. J Biol Chem. 265: 18461-71. PMID: 2170412
4. Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.  |  Hamatake, RK., et al. 1990. J Biol Chem. 265: 4072-83. PMID: 2406268
5. Eukaryotic DNA Replication Fork.  |  Burgers, PMJ. and Kunkel, TA. 2017. Annu Rev Biochem. 86: 417-438. PMID: 28301743
6. Structure of a human replisome shows the organisation and interactions of a DNA replication machine.  |  Jones, ML., et al. 2021. EMBO J. 40: e108819. PMID: 34694004
7. Extrinsic proofreading.  |  Zhou, ZX. and Kunkel, TA. 2022. DNA Repair (Amst). 117: 103369. PMID: 35850061
8. In silico protein interaction screening uncovers DONSON's role in replication initiation.  |  Lim, Y., et al. 2023. Science. 381: eadi3448. PMID: 37590370
9. Asymmetric distribution of parental H3K9me3 in S phase silences L1 elements.  |  Li, Z., et al. 2023. Nature. 623: 643-651. PMID: 37938774
10. Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure.  |  Lee, MY., et al. 1984. Biochemistry. 23: 1906-13. PMID: 6426510
11. Requirement of the yeast RTH1 5' to 3' exonuclease for the stability of simple repetitive DNA.  |  Johnson, RE., et al. 1995. Science. 269: 238-40. PMID: 7618086
12. Regulation of human DNA polymerase delta during the cell cycle.  |  Zeng, XR., et al. 1994. J Biol Chem. 269: 24027-33. PMID: 7929054

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA pol ε A Antibody (3C5.1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA pol ε A Antibody (3C5.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA pol ε A Antibody (3C5.1) Specifications

Alternate Product Name: DNA pol ε A

Product Clone Name: 3C5.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: DNA pol ε A

Epitope Species: human

Product Reactivity/Detection: DNA pol ε A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for DNA pol ε A Antibody (3C5.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin

The preferred DNA pol ε A antibody is DNA pol ε A Antibody (D-10): sc-390785.