DNA pol δ cat Antibody (H-8): sc-374025

DNA pol δ cat Antibody (H-8) is a mouse monoclonal IgG2a antibody that detects DNA pol δ cat in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DNA pol δ cat antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA polymerase delta (DNA pol δ) plays a crucial role in DNA replication, recombination, and repair, which are essential processes for maintaining genome stability. DNA pol δ is particularly important for the synthesis of the lagging strand during DNA replication, where DNA pol δ processes Okazaki fragments, ensuring that the newly synthesized DNA is accurate and complete. DNA pol δ is involved in the repair of damaged DNA, excising erroneous or damaged segments to maintain genomic integrity. DNA pol δ is composed of two main subunits, p125 and p50, with p125 directly interacting with the sliding DNA clamp protein PCNA, which enhances processivity during DNA synthesis. DNA pol δ is subject to regulation by various cell cycle proteins, highlighting DNA pol δ′s importance in coordinating DNA synthesis with the cell cycle. Understanding DNA pol δ function and regulation is vital for elucidating the mechanisms of DNA replication and repair, which have significant implications in cancer research and therapeutic development.

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DNA pol δ cat Antibody (H-8) References:

1. Effect of exogenous MSH6 and POLD1 expression on the mutation rate of the HPRT locus in a human colon cancer cell line with mutator phenotype, DLD-1.  |  Yabuta, T., et al. 2004. Int J Oncol. 24: 697-702. PMID: 14767555
2. Detection of Allelic Variants of the POLE and POLD1 Genes in Colorectal Cancer Patients.  |  LA, P., et al. 2017. Balkan J Med Genet. 20: 83-88. PMID: 29876237
3. POLD1 and POLE Gene Mutations in Jewish Cohorts of Early-Onset Colorectal Cancer and of Multiple Colorectal Adenomas.  |  Rosner, G., et al. 2018. Dis Colon Rectum. 61: 1073-1079. PMID: 30086056
4. Low frequency of POLD1 and POLE exonuclease domain variants in patients with multiple colorectal polyps.  |  Elsayed, FA., et al. 2019. Mol Genet Genomic Med. 7: e00603. PMID: 30827058
5. POLD1 Deficiency Reveals a Role for POLD1 in DNA Repair and T and B Cell Development.  |  Nichols-Vinueza, DX., et al. 2021. J Clin Immunol. 41: 270-273. PMID: 33140240
6. Functional analysis of POLD1 p.ser605del variant: the aging phenotype of MDPL syndrome is associated with an impaired DNA repair capacity.  |  Murdocca, M., et al. 2021. Aging (Albany NY). 13: 4926-4945. PMID: 33618333
7. Resveratrol Mediates the Apoptosis of Triple Negative Breast Cancer Cells by Reducing POLD1 Expression.  |  Liang, ZJ., et al. 2021. Front Oncol. 11: 569295. PMID: 33747905
8. Tumor-Infiltrating Lymphocytes, Tumor Mutational Burden, and Genetic Alterations in Microsatellite Unstable, Microsatellite Stable, or Mutant POLE/POLD1 Colon Cancer.  |  Keshinro, A., et al. 2021. JCO Precis Oncol. 5: PMID: 34250404
9. Investigation of Functional Synergism of CENPF and FOXM1 Identifies POLD1 as Downstream Target in Hepatocellular Carcinoma.  |  Ho, DW., et al. 2022. Front Med (Lausanne). 9: 860395. PMID: 35865168
10. The human POLD1 gene. Identification of an upstream activator sequence, activation by Sp1 and Sp3, and cell cycle regulation.  |  Zhao, L. and Chang, LS. 1997. J Biol Chem. 272: 4869-82. PMID: 9030545

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA pol δ cat Antibody (H-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA pol δ cat Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA pol δ cat Antibody (H-8) Specifications

Alternate Product Name: DNA pol δ cat

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a lambda light chain

Amino Acids: 1079-1116

Epitope Target: DNA pol δ cat

Epitope Species: human

Product Reactivity/Detection: DNA pol δ cat

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for DNA pol δ cat Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred DNA pol δ cat antibody is DNA pol δ cat Antibody (A-9): sc-17776.