DNA pol delta cat Antibody (H-8) recommended for detection of DNA pol δ cat of mouse, rat and human origin by WB, IP, IF and ELISA

DNA pol δ cat Antibody (H-8): sc-374025

DNA pol δ cat Antibody (H-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • DNA pol δ cat Antibody (H-8) is a mouse monoclonal IgG2a (lambda light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1079-1116 near the C-terminus of DNA pol δ cat of human origin
    • Anti-DNA pol delta cat Antibody (H-8) is recommended for detection of DNA pol δ cat of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
    • Anti-DNA pol delta cat Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DNA pol δ cat (H-8): sc-374025.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred detection reagent for DNA pol δ cat Antibody (H-8) for WB applications. This reagent is now offered in a bundle with DNA pol δ cat Antibody (H-8) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I used this antibody for WB in DN3A4(mouse NKT cell line) and Jurkat (human T cell line) whole cell lysates but there was no band. Beta-actin band was confirmed. Which cell line could be a positive control? Is there a possibility of antibody problem?

Asked by: wmtmaksm
Thank you for your question. We recommend using HeLa, HL-60, MCF7 or MOLT-4 whole cell lysates as positive controls with this antibodies. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-08

What application is the blocking peptide sc-374025 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from GREAT Great. This antibody is very good for IF staining.
Date published: 2017-08-17
Rated 5 out of 5 by from Works well for WB with in NIH/3T3 nuclear extract Works well for WB with in NIH/3T3 nuclear extract. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using in HeLa nuclear extract. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Produced superb immunofluorescence nuclear Produced superb immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-03-01
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