DNA pol δ cat Antibody (A-9): sc-17776

DNA pol δ cat Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects DNA pol delta cat protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-DNA pol δ cat antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA pol delta cat protein plays a crucial role in DNA replication, recombination, and repair, which are essential processes for maintaining genome stability. Specifically, DNA pol δ cat is involved in the synthesis of the lagging strand during DNA replication and processes Okazaki fragments. DNA pol δ cat consists of two subunits, p125 and p50, with p125 directly interacting with the sliding DNA clamp protein PCNA, facilitating efficient DNA synthesis. DNA pol δ cat is regulated by various cell cycle proteins, ensuring that activity is tightly controlled during cell division. Understanding DNA pol δ cat function and regulation is vital, as dysregulation can lead to genomic instability and contribute to the development of various diseases, including cancer.

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DNA pol δ cat Antibody (A-9) References:

1. Direct interaction of proliferating cell nuclear antigen with the p125 catalytic subunit of mammalian DNA polymerase delta.  |  Zhang, P., et al. 1999. J Biol Chem. 274: 26647-53. PMID: 10480866
2. A third essential DNA polymerase in S. cerevisiae.  |  Morrison, A., et al. 1990. Cell. 62: 1143-51. PMID: 2169349
3. Discontinuous DNA synthesis by purified mammalian proteins.  |  Goulian, M., et al. 1990. J Biol Chem. 265: 18461-71. PMID: 2170412
4. Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas.  |  Palles, C., et al. 2013. Nat Genet. 45: 136-44. PMID: 23263490
5. Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.  |  Hamatake, RK., et al. 1990. J Biol Chem. 265: 4072-83. PMID: 2406268
6. Role of POLE and POLD1 in familial cancer.  |  Mur, P., et al. 2020. Genet Med. 22: 2089-2100. PMID: 32792570
7. POLE/POLD1 mutation and tumor immunotherapy.  |  Ma, X., et al. 2022. J Exp Clin Cancer Res. 41: 216. PMID: 35780178
8. Functional landscapes of POLE and POLD1 mutations in checkpoint blockade-dependent antitumor immunity.  |  Ma, X., et al. 2022. Nat Genet. 54: 996-1012. PMID: 35817971
9. DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC.  |  Wang, Y., et al. 2023. Nat Commun. 14: 2421. PMID: 37105989
10. Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure.  |  Lee, MY., et al. 1984. Biochemistry. 23: 1906-13. PMID: 6426510
11. Requirement of the yeast RTH1 5' to 3' exonuclease for the stability of simple repetitive DNA.  |  Johnson, RE., et al. 1995. Science. 269: 238-40. PMID: 7618086
12. Regulation of human DNA polymerase delta during the cell cycle.  |  Zeng, XR., et al. 1994. J Biol Chem. 269: 24027-33. PMID: 7929054

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA pol δ cat Antibody (A-9) has 36 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA pol δ cat Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA pol δ cat Antibody (A-9) Specifications

Alternate Product Name: POLD1

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: DNA pol δ cat

Epitope Species: human

Product Reactivity/Detection: DNA pol δ cat

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 36 citations in various scientific publications

Available Conjugates/Forms for DNA pol δ cat Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin