DNA pol δ 2 Antibody (D-7): sc-390583

DNA pol δ 2 Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects DNA pol delta 2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-DNA pol δ 2 antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA pol delta 2 protein plays a crucial role in DNA replication, recombination, and repair, which are essential processes for maintaining genome stability. Specifically, DNA pol δ is involved in the synthesis of the lagging strand during DNA replication, where DNA pol δ processes Okazaki fragments, and participates in DNA repair mechanisms by excising damaged DNA and correcting mismatches. DNA pol delta 2 protein is composed of two subunits, p125 and p50, with p125 directly interacting with the sliding clamp protein PCNA, which is vital for enhancing polymerase processivity. DNA pol δ can be regulated by various cell cycle proteins, ensuring that DNA pol δ activity is tightly controlled during different phases of the cell cycle. Understanding the function and regulation of DNA pol δ is important for elucidating the mechanisms of genomic stability and potential implications in cancer biology, where dysregulation of DNA repair pathways can lead to tumorigenesis.

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DNA pol δ 2 Antibody (D-7) References:

1. Direct interaction of proliferating cell nuclear antigen with the p125 catalytic subunit of mammalian DNA polymerase delta.  |  Zhang, P., et al. 1999. J Biol Chem. 274: 26647-53. PMID: 10480866
2. A third essential DNA polymerase in S. cerevisiae.  |  Morrison, A., et al. 1990. Cell. 62: 1143-51. PMID: 2169349
3. Discontinuous DNA synthesis by purified mammalian proteins.  |  Goulian, M., et al. 1990. J Biol Chem. 265: 18461-71. PMID: 2170412
4. Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.  |  Hamatake, RK., et al. 1990. J Biol Chem. 265: 4072-83. PMID: 2406268
5. Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure.  |  Lee, MY., et al. 1984. Biochemistry. 23: 1906-13. PMID: 6426510
6. Requirement of the yeast RTH1 5' to 3' exonuclease for the stability of simple repetitive DNA.  |  Johnson, RE., et al. 1995. Science. 269: 238-40. PMID: 7618086
7. Regulation of human DNA polymerase delta during the cell cycle.  |  Zeng, XR., et al. 1994. J Biol Chem. 269: 24027-33. PMID: 7929054

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA pol δ 2 Antibody (D-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA pol δ 2 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA pol δ 2 Antibody (D-7) Specifications

Alternate Product Name: DNA pol δ 2

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 448-473

Epitope Target: DNA pol δ ss

Epitope Species: human

Product Reactivity/Detection: DNA pol δ 2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for DNA pol δ 2 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin