DNA-PKCS Antibody (G-12): sc-390849

DNA-PKCS Antibody (G-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects DNA-PKcs in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-DNA-PKcs antibody (G-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA-PKcs plays a crucial role in cellular response to DNA double-strand breaks (DSBs), which are among the most lethal forms of DNA damage. DNA-PKcs is primarily located in the nucleus, where DNA-PKcs is essential for the non-homologous end joining (NHEJ) repair pathway, a critical mechanism for maintaining genomic stability. Deficiencies in DNA-PKcs can lead to severe consequences, including increased susceptibility to cancer and other genetic disorders, as the inability to repair DSBs can result in chromosomal aberrations and cell death. DNA-PKcs is part of a larger complex that includes the Ku proteins, which are vital for recognizing and binding to DSBs, thereby facilitating the repair process. The significance of DNA-PKcs in the NHEJ pathway underscores DNA-PKcs potential as a therapeutic target in cancer treatment, where enhancing or inhibiting DNA-PKcs activity could influence tumor response to radiation and chemotherapy.

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DNA-PKCS Antibody (G-12) References:

1. Synthetically Lethal Interactions of ATM, ATR, and DNA-PKcs.  |  Kantidze, OL., et al. 2018. Trends Cancer. 4: 755-768. PMID: 30352678
2. Structural insights into the critical DNA damage sensors DNA-PKcs, ATM and ATR.  |  Baretic, D., et al. 2019. Prog Biophys Mol Biol. 147: 4-16. PMID: 31255703
3. DNA-PKcs Ser2056 auto-phosphorylation is affected by an O-GlcNAcylation/phosphorylation interplay.  |  Lafont, F., et al. 2020. Biochim Biophys Acta Gen Subj. 1864: 129705. PMID: 32805318
4. DNA-PKcs interacts with and phosphorylates Fis1 to induce mitochondrial fragmentation in tubular cells during acute kidney injury.  |  Wang, S., et al. 2022. Sci Signal. 15: eabh1121. PMID: 35290083
5. Purification of DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PKcs) from HeLa Cells.  |  Lee, L., et al. 2022. Methods Mol Biol. 2444: 227-241. PMID: 35290641
6. SENP3-mediated TIP60 deSUMOylation is required for DNA-PKcs activity and DNA damage repair.  |  Han, Y., et al. 2022. MedComm (2020). 3: e123. PMID: 35356800
7. Chemical inhibition of DNA-PKcs impairs the activation and cytotoxicity of CD4+ helper and CD8+ effector T cells.  |  Azevedo-Pouly, AC., et al. 2023. Immunol Cell Biol. 101: 663-671. PMID: 37149747
8. DNA-PKcs-mediated transcriptional regulation of TOP2B drives chemoresistance in acute myeloid leukemia.  |  Mishra, SV., et al. 2024. J Cell Sci. 137: PMID: 38240344
9. Quercetin inhibits necroptosis in cardiomyocytes after ischemia-reperfusion via DNA-PKcs-SIRT5-orchestrated mitochondrial quality control.  |  Chang, X., et al. 2024. Phytother Res. 38: 2496-2517. PMID: 38447978
10. ER-associated degradation ligase HRD1 links ER stress to DNA damage repair by modulating the activity of DNA-PKcs.  |  Xiang, Z., et al. 2024. Proc Natl Acad Sci U S A. 121: e2403038121. PMID: 39226359

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA-PKCS Antibody (G-12) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA-PKCS Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA-PKCS Antibody (G-12) Specifications

Alternate Product Name: DNA-PKCS

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 4100-4128

Epitope Target: DNA-PKCS

Epitope Species: human

Product Reactivity/Detection: DNA-PKcs

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for DNA-PKCS Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred DNA-PK_CS antibody is DNA-PK_CS Antibody (G-4): sc-5282.