DNA Ligase IV Antibody (D-8): sc-271299

DNA Ligase IV Antibody (D-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects DNA Ligase IV of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA Ligase IV (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA Ligase IV plays a crucial role in repairing double-strand breaks in DNA, which is vital for maintaining genomic stability and preventing mutations that can lead to cancer. DNA Ligase IV operates with X-ray repair cross-complementing protein XRCC4, forming a complex that enhances DNA repair efficiency by preferentially binding to DNA with nicks or broken ends. This interaction is particularly important during V(D)J recombination, a process essential for adaptive immune system development, where double-strand breaks are generated to facilitate immunoglobulin and T-cell receptor gene rearrangement. Disruptions in DNA Ligase IV or XRCC4 function can lead to severe consequences, including increased apoptosis in developing neurons and impaired maturation of B and T cells, highlighting DNA Ligase IV′s importance in both DNA repair and immune system development.

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DNA Ligase IV Antibody (D-8) References:

1. Absence of DNA ligase IV protein in XR-1 cells: evidence for stabilization by XRCC4.  |  Bryans, M., et al. 1999. Mutat Res. 433: 53-8. PMID: 10047779
2. DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity.  |  Modesti, M., et al. 1999. EMBO J. 18: 2008-18. PMID: 10202163
3. A new gene involved in DNA double-strand break repair and V(D)J recombination is located on human chromosome 10p.  |  Moshous, D., et al. 2000. Hum Mol Genet. 9: 583-8. PMID: 10699181
4. Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.  |  Chen, L., et al. 2000. J Biol Chem. 275: 26196-205. PMID: 10854421
5. DNA ligase IV and XRCC4 form a stable mixed tetramer that functions synergistically with other repair factors in a cell-free end-joining system.  |  Lee, KJ., et al. 2000. J Biol Chem. 275: 34787-96. PMID: 10945980
6. Crystal structure of the Xrcc4 DNA repair protein and implications for end joining.  |  Junop, MS., et al. 2000. EMBO J. 19: 5962-70. PMID: 11080143
7. Knockdown of DNA ligase IV/XRCC4 by RNA interference inhibits herpes simplex virus type I DNA replication.  |  Muylaert, I. and Elias, P. 2007. J Biol Chem. 282: 10865-72. PMID: 17296606
8. Length-dependent binding of human XLF to DNA and stimulation of XRCC4.DNA ligase IV activity.  |  Lu, H., et al. 2007. J Biol Chem. 282: 11155-62. PMID: 17317666
9. Epstein-Barr virus-associated B-cell lymphoma in a patient with DNA ligase IV (LIG4) syndrome.  |  Toita, N., et al. 2007. Am J Med Genet A. 143A: 742-5. PMID: 17345618
10. DNA ligase IV deficiency: Immunoglobulin class deficiency depends on the genotype.  |  Dard, R., et al. 2017. Pediatr Allergy Immunol. 28: 298-303. PMID: 28039949
11. Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.  |  Chen, SH. and Yu, X. 2019. Nucleic Acids Res. 47: 1321-1334. PMID: 30496552
12. Catalytically inactive DNA ligase IV promotes DNA repair in living cells.  |  Goff, NJ., et al. 2022. Nucleic Acids Res. 50: 11058-11071. PMID: 36263813

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA Ligase IV Antibody (D-8) has 29 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA Ligase IV Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA Ligase IV Antibody (D-8) Specifications

Alternate Product Name: DNA Ligase 4

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 545-844

Epitope Target: DNA Ligase IV

Epitope Species: human

Product Reactivity/Detection: DNA Ligase IV

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for DNA Ligase IV Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin