DNA Ligase I Antibody (10H5): sc-56087
- DNA Ligase I Antibody (10H5) is a mouse monoclonal IgG1, cited in 3 publications, provided at 50 µg/0.5 ml
- raised against amino acids 1-919 of DNA Ligase I of human origin
- recommended for detection of DNA Ligase I of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DNA Ligase I Antibody (10H5). This work is in progress.
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DNA Ligase I Antibody (10H5) is a mouse monoclonal IgG1 antibody that detects DNA Ligase I in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). DNA Ligase I is crucial for maintaining genome integrity, playing a vital role in DNA replication and repair by joining Okazaki fragments during lagging strand synthesis and sealing nicks in DNA during excision repair processes. DNA Ligase I is predominantly located in the nucleus, where DNA Ligase I activity is essential for proper DNA repair pathways, ensuring efficient repair of DNA breaks to prevent genomic instability. Notably, elevated levels of DNA Ligase I have been observed in various human tumors compared to benign tissues, highlighting DNA Ligase I potential as a biomarker for cancer progression and a target for therapeutic intervention. Additionally, DNA Ligase I antisense oligonucleotides have been shown to reduce tumor cell proliferation, suggesting a significant role in cancer biology. Understanding DNA Ligase I function and localization is critical, as DNA Ligase I dysregulation can lead to severe consequences, including chromosomal breakage disorders such as Bloom′s syndrome, where patients exhibit altered DNA Ligase I activity. anti-DNA Ligase I antibody (10H5) is an invaluable tool for researchers investigating DNA repair mechanisms and DNA Ligase I implications in cancer research.
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DNA Ligase I Antibody (10H5) References:
- Induction of DNA ligase I by 1-beta-D-arabinosylcytosine and aphidicolin in MiaPaCa human pancreatic cancer cells. | Sun, D., et al. 2002. Exp Cell Res. 280: 90-6. PMID: 12372342
- Human DNA ligase I completely encircles and partially unwinds nicked DNA. | Pascal, JM., et al. 2004. Nature. 432: 473-8. PMID: 15565146
- Activation of mammalian DNA ligase I through phosphorylation by casein kinase II. | Prigent, C., et al. 1992. EMBO J. 11: 2925-33. PMID: 1639065
- Differential recruitment of DNA Ligase I and III to DNA repair sites. | Mortusewicz, O., et al. 2006. Nucleic Acids Res. 34: 3523-32. PMID: 16855289
- DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories. | Vago, R., et al. 2009. Cell Cycle. 8: 2600-7. PMID: 19597347
- Defects in DNA ligase I trigger PCNA ubiquitylation at Lys 107. | Das-Bradoo, S., et al. 2010. Nat Cell Biol. 12: 74-9; sup pp 1-20. PMID: 20010813
- Structure-activity relationships among DNA ligase inhibitors: Characterization of a selective uncompetitive DNA ligase I inhibitor. | Howes, TRL., et al. 2017. DNA Repair (Amst). 60: 29-39. PMID: 29078112
- Recent advances in the targeting of human DNA ligase I as a potential new strategy for cancer treatment. | Saquib, M., et al. 2019. Eur J Med Chem. 182: 111657. PMID: 31499361
- DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage. | Tang, Q., et al. 2020. Mutagenesis. 35: 391-404. PMID: 32914844
- DNA ligase I fidelity mediates the mutagenic ligation of pol β oxidized and mismatch nucleotide insertion products in base excision repair. | Kamble, P., et al. 2021. J Biol Chem. 296: 100427. PMID: 33600799
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA Ligase I Antibody (10H5) | sc-56087 | 50 µg/0.5 ml | $322.00 |