DLK2 Antibody (B-10): sc-376896
- DLK2 Antibody (B-10) is a mouse monoclonal IgG2a κ DLK2 antibody provided at 200 µg/ml
- raised against amino acids 181-305 mapping within an N-terminal extracellular domain of DLK2 of human origin
- DLK2 Antibody (B-10) is recommended for detection of DLK2 of human origin by WB, IP, IF and ELISA
- Anti-DLK2 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DLK2 Antibody (B-10) for WB applications. These reagents are now offered in bundles with DLK2 Antibody (B-10) (see ordering information below).
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DLK2 Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects DLK2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DLK2 (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DLK2 protein, also known as delta homolog 2 or epidermal growth factor-like protein 9, is a 383 amino acid single-pass transmembrane protein characterized by six tandem epidermal growth factor-like repeats in its extracellular domain, which is significant for its role in cell signaling and interaction with other proteins. DLK2 protein is primarily located in the cell membrane, where DLK2 plays a crucial role in mediating cellular communication and influencing processes such as adipogenesis. Notably, DLK2 has been shown to have an inverse relationship with DLK homolog, suggesting that the balance of these proteins is essential for proper adipocyte differentiation. The coordinated expression of DLK and DLK2 is vital, as alterations in the levels of one can significantly impact the expression of the other, thereby modulating adipogenesis and potentially influencing metabolic disorders. Additionally, two isoforms of DLK2 arise from alternative splicing, which may contribute to functional diversity and regulatory mechanisms in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DLK2 Antibody (B-10) References:
- Adipocyte differentiation is modulated by secreted delta-like (dlk) variants and requires the expression of membrane-associated dlk. | Garcés, C., et al. 1999. Differentiation. 64: 103-14. PMID: 10234807
- Molecular regulation of adipocyte differentiation. | Cowherd, RM., et al. 1999. Semin Cell Dev Biol. 10: 3-10. PMID: 10355023
- Adipocyte differentiation and gene expression. | Ntambi, JM. and Young-Cheul, K. 2000. J Nutr. 130: 3122S-3126S. PMID: 11110885
- The novel gene EGFL9/Dlk2, highly homologous to Dlk1, functions as a modulator of adipogenesis. | Nueda, ML., et al. 2007. J Mol Biol. 367: 1270-80. PMID: 17320102
- The EGF-like protein dlk1 inhibits notch signaling and potentiates adipogenesis of mesenchymal cells. | Nueda, ML., et al. 2007. J Mol Biol. 367: 1281-93. PMID: 17320900
- dlk1 specifically interacts with insulin-like growth factor binding protein 1 to modulate adipogenesis of 3T3-L1 cells. | Nueda, ML., et al. 2008. J Mol Biol. 379: 428-42. PMID: 18466921
- Minireview: Pref-1: role in adipogenesis and mesenchymal cell fate. | Sul, HS. 2009. Mol Endocrinol. 23: 1717-25. PMID: 19541743
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DLK2 Antibody (B-10) | sc-376896 | 200 µg/ml | $322.00 | |||
DLK2 Antibody (B-10): m-IgGκ BP-HRP Bundle | sc-523130 | 200 µg Ab, 40 µg BP | $361.00 | |||
DLK2 Antibody (B-10): m-IgG2a BP-HRP Bundle | sc-546144 | 200 µg Ab; 10 µg BP | $361.00 | |||
DLK2 Antibody (B-10) AC | sc-376896 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DLK2 Antibody (B-10) HRP | sc-376896 HRP | 200 µg/ml | $322.00 | |||
DLK2 Antibody (B-10) FITC | sc-376896 FITC | 200 µg/ml | $336.00 | |||
DLK2 Antibody (B-10) PE | sc-376896 PE | 200 µg/ml | $349.00 | |||
DLK2 Antibody (B-10) Alexa Fluor® 488 | sc-376896 AF488 | 200 µg/ml | $364.00 | |||
DLK2 Antibody (B-10) Alexa Fluor® 546 | sc-376896 AF546 | 200 µg/ml | $364.00 | |||
DLK2 Antibody (B-10) Alexa Fluor® 594 | sc-376896 AF594 | 200 µg/ml | $364.00 | |||
DLK2 Antibody (B-10) Alexa Fluor® 647 | sc-376896 AF647 | 200 µg/ml | $364.00 | |||
DLK2 Antibody (B-10) Alexa Fluor® 680 | sc-376896 AF680 | 200 µg/ml | $364.00 | |||
DLK2 Antibody (B-10) Alexa Fluor® 790 | sc-376896 AF790 | 200 µg/ml | $364.00 |