Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DIO1 CRISPR/Cas9 KO Plasmid (m): sc-420002
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DIO1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DIO1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DIO1 Antibody (B-7): sc-515198
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DIO1 CRISPR/Cas9 KO Plasmid (m) | sc-420002 | 20 µg | $397.00 |
Overview
Mouse Dio1 encodes iodothyronine deiodinase 1 (DIO1), a selenoenzyme that catalyzes outer- and inner-ring deiodination of thyroid hormones, contributing to peripheral conversion of thyroxine (T4) to bioactive triiodothyronine (T3) and to hormone clearance. DIO1 activity supports endocrine and metabolic homeostasis by shaping intracellular and circulating thyroid hormone availability, thereby influencing transcriptional programs controlled by thyroid hormone receptors. Dio1 is prominently studied in the context of hepatic and renal thyroid hormone metabolism, selenium biology, and redox-dependent enzyme function. Altered deiodinase expression or activity has been associated with dysregulated thyroid hormone signaling in metabolic and inflammatory states, motivating mechanistic studies of Dio1-dependent pathways in mouse models.
DIO1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dio1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dio1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dio1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DIO1 protein expression.
This CRISPR knockout system enables efficient generation of Dio1-deficient cell models for investigation of DIO1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dio1 exon(s) critical for DIO1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dio1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DIO1 CRISPR/Cas9 KO Plasmid (m) and DIO1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dio1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DIO1 HDR Plasmid (m) and DIO1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dio1 homology arms to support homology-directed repair at defined Dio1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.