dinitrophenyl Antibody (10.12): sc-69698
- dinitrophenyl Antibody (10.12) is a mouse monoclonal IgG2b dinitrophenyl antibody provided at 200 µg/ml
- dinitrophenyl Antibody (10.12) is recommended for detection of dinitrophenyl-γ2b by ELISA
- Anti-dinitrophenyl Antibody (10.12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for dinitrophenyl Antibody (10.12). These reagents are now offered in bundles with dinitrophenyl Antibody (10.12) (see ordering information below).
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Dinitrophenyl Antibody (10.12) is a mouse monoclonal IgG2b antibody that detects dinitrophenyl protein in non-species specific samples by enzyme-linked immunosorbent assay (ELISA). Anti-dinitrophenyl antibody (10.12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dinitrophenyl (DNP) is a synthetic compound that acts as a cellular metabolic poison by functioning as a proton ionophore, disrupting oxidative phosphorylation through proton transport across mitochondrial membranes. This disruption causes rapid energy depletion without adenosine triphosphate (ATP) production, converting proton gradient energy into heat. DNP′s importance extends beyond environmental concerns from automobile emissions and industrial processes to scientific research, where DNP serves as a valuable tool in biochemistry studies examining bioenergetic regulation across organisms, offering insights into metabolic processes and potential therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
dinitrophenyl Antibody (10.12) References:
- Conventional detection of 2,4-dinitrophenol using quartz crystal microbalance. | Park, JW., et al. 2003. IEEE Trans Ultrason Ferroelectr Freq Control. 50: 193-5. PMID: 12625592
- A reevaluation of the mechanisms involved in the secretion of catecholamine evoked by 2,4-dinitrophenol from chemoreceptor cells of the rabbit carotid body. | Rocher, A., et al. 2003. Adv Exp Med Biol. 536: 85-93. PMID: 14635653
- The mitochondrial uncoupler 2,4-dinitrophenol attenuates tissue damage and improves mitochondrial homeostasis following transient focal cerebral ischemia. | Korde, AS., et al. 2005. J Neurochem. 94: 1676-84. PMID: 16045446
- Aqueous atmospheric chemistry: formation of 2,4-dinitrophenol upon nitration of 2-nitrophenol and 4-nitrophenol in solution. | Vione, D., et al. 2005. Environ Sci Technol. 39: 7921-31. PMID: 16295857
- Novel neuroprotective, neuritogenic and anti-amyloidogenic properties of 2,4-dinitrophenol: the gentle face of Janus. | De Felice, FG. and Ferreira, ST. 2006. IUBMB Life. 58: 185-91. PMID: 16754295
- Two deaths attributed to the use of 2,4-dinitrophenol. | Miranda, EJ., et al. 2006. J Anal Toxicol. 30: 219-22. PMID: 16803658
- Targeting dinitrophenol to mitochondria: limitations to the development of a self-limiting mitochondrial protonophore. | Blaikie, FH., et al. 2006. Biosci Rep. 26: 231-43. PMID: 16850251
- Effects of ADP-ribosylation of GTP-binding protein by pertussis toxin on immunoglobulin E-dependent and -independent histamine release from mast cells and basophils. | Saito, H., et al. 1987. J Immunol. 138: 3927-34. PMID: 2438330
- The stimuli releasing histamine from murine bone marrow-derived mast cells. 1. The presence of P2-purinoceptors. | Nakamura, H., et al. 1989. Arerugi. 38: 1359-63. PMID: 2483881
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
dinitrophenyl Antibody (10.12) | sc-69698 | 200 µg/ml | $322.00 | |||
dinitrophenyl Antibody (10.12): m-IgG Fc BP-HRP Bundle | sc-525691 | 200 µg Ab; 10 µg BP | $361.00 | |||
dinitrophenyl Antibody (10.12): m-IgG2b BP-HRP Bundle | sc-549620 | 200 µg Ab; 10 µg BP | $361.00 | |||
dinitrophenyl Antibody (10.12) AC | sc-69698 AC | 500 µg/ml, 25% agarose | $424.00 | |||
dinitrophenyl Antibody (10.12) HRP | sc-69698 HRP | 200 µg/ml | $322.00 | |||
dinitrophenyl Antibody (10.12) FITC | sc-69698 FITC | 200 µg/ml | $336.00 | |||
dinitrophenyl Antibody (10.12) PE | sc-69698 PE | 200 µg/ml | $349.00 | |||
dinitrophenyl Antibody (10.12) Alexa Fluor® 488 | sc-69698 AF488 | 200 µg/ml | $364.00 | |||
dinitrophenyl Antibody (10.12) Alexa Fluor® 546 | sc-69698 AF546 | 200 µg/ml | $364.00 | |||
dinitrophenyl Antibody (10.12) Alexa Fluor® 594 | sc-69698 AF594 | 200 µg/ml | $364.00 | |||
dinitrophenyl Antibody (10.12) Alexa Fluor® 647 | sc-69698 AF647 | 200 µg/ml | $364.00 | |||
dinitrophenyl Antibody (10.12) Alexa Fluor® 680 | sc-69698 AF680 | 200 µg/ml | $364.00 | |||
dinitrophenyl Antibody (10.12) Alexa Fluor® 790 | sc-69698 AF790 | 200 µg/ml | $364.00 |