DinB Antibody (A-9): sc-166667
- DinB Antibody (A-9) is a mouse monoclonal IgG1 κ DinB antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 131-310 mapping near the N-terminus of DinB of human origin
- DinB Antibody (A-9) is recommended for detection of all DinB isoforms of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DinB Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-166667 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DinB Antibody (A-9) for WB applications. These reagents are now offered in bundles with DinB Antibody (A-9) (see ordering information below).
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DinB Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects DinB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DinB antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DinB protein, also known as DNA polymerase IV or DNA polymerase k, is crucial for maintaining genomic stability, particularly during DNA replication under stress conditions. DinB functions as an SOS-inducible, error-prone polymerase that facilitates translesion DNA synthesis, allowing cells to bypass DNA lesions that would otherwise stall replication forks. This mechanism helps prevent accumulation of potentially harmful mutations arising from replication stress, playing a significant role in DNA damage-induced mutagenesis. DinB shows high expression in adrenal cortex and testis, with expression regulated by tumor suppressor protein p53, which responds to DNA-damaging agents by upregulating DinB. Understanding DinB′s role in DNA repair mechanisms remains essential for elucidating pathways that contribute to genomic integrity and potential implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DinB Antibody (A-9) References:
- Crystal structure of a DinB lesion bypass DNA polymerase catalytic fragment reveals a classic polymerase catalytic domain. | Zhou, BL., et al. 2001. Mol Cell. 8: 427-37. PMID: 11545744
- Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus. | Silvian, LF., et al. 2001. Nat Struct Biol. 8: 984-9. PMID: 11685247
- Constitutive and regulated expression of the mouse Dinb (Polkappa) gene encoding DNA polymerase kappa. | Velasco-Miguel, S., et al. 2003. DNA Repair (Amst). 2: 91-106. PMID: 12509270
- The dinB operon and spontaneous mutation in Escherichia coli. | McKenzie, GJ., et al. 2003. J Bacteriol. 185: 3972-7. PMID: 12813093
- SOS-independent induction of dinB transcription by beta-lactam-mediated inhibition of cell wall synthesis in Escherichia coli. | Pérez-Capilla, T., et al. 2005. J Bacteriol. 187: 1515-8. PMID: 15687217
- Genomic buffering mitigates the effects of deleterious mutations in bacteria. | Maisnier-Patin, S., et al. 2005. Nat Genet. 37: 1376-9. PMID: 16273106
- Two processivity clamp interactions differentially alter the dual activities of UmuC. | Beuning, PJ., et al. 2006. Mol Microbiol. 59: 460-74. PMID: 16390442
- A single amino acid governs enhanced activity of DinB DNA polymerases on damaged templates. | Jarosz, DF., et al. 2006. Nature. 439: 225-8. PMID: 16407906
- Single-strand-specific exonucleases prevent frameshift mutagenesis by suppressing SOS induction and the action of DinB/DNA polymerase IV in growing cells. | Hersh, MN., et al. 2006. J Bacteriol. 188: 2336-42. PMID: 16547019
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DinB Antibody (A-9) | sc-166667 | 200 µg/ml | $322.00 | |||
DinB Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-529026 | 200 µg Ab; 10 µg BP | $361.00 | |||
DinB Antibody (A-9): m-IgGκ BP-HRP Bundle | sc-521702 | 200 µg Ab, 40 µg BP | $361.00 | |||
DinB Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-543276 | 200 µg Ab; 20 µg BP | $361.00 | |||
DinB Antibody (A-9) X | sc-166667 X | 200 µg/0.1 ml | $322.00 | |||
DinB Antibody (A-9) AC | sc-166667 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DinB Antibody (A-9) HRP | sc-166667 HRP | 200 µg/ml | $322.00 | |||
DinB Antibody (A-9) FITC | sc-166667 FITC | 200 µg/ml | $336.00 | |||
DinB Antibody (A-9) PE | sc-166667 PE | 200 µg/ml | $349.00 | |||
DinB Antibody (A-9) Alexa Fluor® 488 | sc-166667 AF488 | 200 µg/ml | $364.00 | |||
DinB Antibody (A-9) Alexa Fluor® 546 | sc-166667 AF546 | 200 µg/ml | $364.00 | |||
DinB Antibody (A-9) Alexa Fluor® 594 | sc-166667 AF594 | 200 µg/ml | $364.00 | |||
DinB Antibody (A-9) Alexa Fluor® 647 | sc-166667 AF647 | 200 µg/ml | $364.00 | |||
DinB Antibody (A-9) Alexa Fluor® 680 | sc-166667 AF680 | 200 µg/ml | $364.00 | |||
DinB Antibody (A-9) Alexa Fluor® 790 | sc-166667 AF790 | 200 µg/ml | $364.00 |