DICE1 CRISPR/Cas9 KO Plasmid (m): sc-421932
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DICE1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DICE1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DICE1 Antibody (H-6): sc-376524
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DICE1 CRISPR/Cas9 KO Plasmid (m) | sc-421932 | 20 µg | $397.00 |
Overview
Ints6 encodes DICE1, a conserved component of the Integrator complex that associates with RNA polymerase II to coordinate 3′ end processing of small nuclear RNAs and broader transcriptional control. Through its roles in RNA maturation and transcriptional regulation, DICE1 influences cell-cycle progression, differentiation, and genome-wide gene expression programs. Altered INTS6/DICE1 activity has been linked to disrupted growth control and oncogenic signaling contexts, making it relevant for studying mechanisms of tumor suppression and transcriptional stress. In mouse systems, Ints6 perturbation is useful for dissecting Integrator-dependent pathways that couple RNA processing to chromatin and signaling outputs.
DICE1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ints6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ints6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ints6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DICE1 protein expression.
This CRISPR knockout system enables efficient generation of Ints6-deficient cell models for investigation of DICE1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ints6 exon(s) critical for DICE1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ints6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DICE1 CRISPR/Cas9 KO Plasmid (m) and DICE1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ints6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DICE1 HDR Plasmid (m) and DICE1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ints6 homology arms to support homology-directed repair at defined Ints6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.