Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dia 1 CRISPR/Cas9 KO Plasmid (m): sc-420000-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dia 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dia 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dia 1 Antibody (E-4): sc-373807
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dia 1 CRISPR/Cas9 KO Plasmid (m) | sc-420000-KO-2 | 20 µg | $397.00 |
Overview
Diaph1 (mDia1) encodes the formin Dia 1, an actin nucleation and elongation factor that coordinates linear F-actin assembly downstream of Rho family GTPases, including RhoA. Dia 1 supports cytoskeletal remodeling processes such as cell polarization, adhesion, cytokinesis, and directed migration by linking signaling cues to actin dynamics and microtubule stabilization. Through these functions, Diaph1 is frequently studied in pathways governing immune cell trafficking, vascular and epithelial barrier behavior, and mechanotransduction. Altered DIAPH1 activity has been associated with cytoskeleton-related pathophysiology, including platelet and hematologic phenotypes and progressive sensorineural hearing loss, motivating mechanistic studies in mouse systems.
Dia 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Diaph1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Diaph1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Diaph1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dia 1 protein expression.
This CRISPR knockout system enables efficient generation of Diaph1-deficient cell models for investigation of Dia 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Diaph1 exon(s) critical for Dia 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Diaph1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dia 1 CRISPR/Cas9 KO Plasmid (m) and Dia 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Diaph1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dia 1 HDR Plasmid (m) and Dia 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Diaph1 homology arms to support homology-directed repair at defined Diaph1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.