Dia 1 Antibody (E-4): sc-373807

Dia 1 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dia 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Dia 1 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dia 1 protein, also known as DIAPH1 (diaphanous homolog 1) or DRF1, plays a crucial role in regulating actin dynamics, which is essential for various cellular processes such as cell shape, motility, and division. DIAPH1 is characterized by unique structure, including tandemly aligned formin homology 1 (FH1) and formin homology 2 (FH2) domains that facilitate nucleation and elongation of actin filaments. DIAPH1 contains a diaphanous autoregulatory domain (DAD) that modulates activity through autoinhibition, as well as a Rho GTPase-binding/formin homology 3 (GBD/FH3) domain that interacts with DAD domain. DIAPH1 localization to cell membrane allows direct regulation of actin polymerization at sites of cellular activity. Mutations in DIAPH1 gene have been associated with deafness autosomal dominant type 1 (DFNA1), highlighting DIAPH1′s importance in auditory function.

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Dia 1 Antibody (E-4) References:

1. Distinct actions and cooperative roles of ROCK and mDia in Rho small G protein-induced reorganization of the actin cytoskeleton in Madin-Darby canine kidney cells.  |  Nakano, K., et al. 1999. Mol Biol Cell. 10: 2481-91. PMID: 10436006
2. Cooperation between mDia1 and ROCK in Rho-induced actin reorganization.  |  Watanabe, N., et al. 1999. Nat Cell Biol. 1: 136-43. PMID: 10559899
3. Diaphanous-related formins bridge Rho GTPase and Src tyrosine kinase signaling.  |  Tominaga, T., et al. 2000. Mol Cell. 5: 13-25. PMID: 10678165
4. Functional analysis of the Drosophila diaphanous FH protein in early embryonic development.  |  Afshar, K., et al. 2000. Development. 127: 1887-97. PMID: 10751177
5. The receptor for advanced glycation end products (RAGE) and DIAPH1: unique mechanisms and healing the wounded vascular system.  |  Ramasamy, R., et al. 2019. Expert Rev Proteomics. 16: 471-474. PMID: 30324836
6. Association of DIAPH1 gene polymorphisms with ischemic stroke.  |  Ren, Z., et al. 2020. Aging (Albany NY). 12: 416-435. PMID: 31899686
7. DIAPH1 regulates chromosomal instability of cancer cells by controlling microtubule dynamics.  |  Miao, S., et al. 2021. Eur J Cell Biol. 100: 151156. PMID: 33689956
8. DIAPH1 Promotes Laryngeal Squamous Cell Carcinoma Progression Through Cell Cycle Regulation.  |  Yang, J., et al. 2021. Front Oncol. 11: 716876. PMID: 34631544
9. Diaph1 knockout inhibits mouse primordial germ cell proliferation and affects gonadal development.  |  Zhao, X., et al. 2024. Reprod Biol Endocrinol. 22: 82. PMID: 39010074
10. Nonsyndromic deafness DFNA1 associated with mutation of a human homolog of the Drosophila gene diaphanous.  |  Lynch, ED., et al. 1997. Science. 278: 1315-8. PMID: 9360932
11. A human homologue of the Drosophila melanogaster diaphanous gene is disrupted in a patient with premature ovarian failure: evidence for conserved function in oogenesis and implications for human sterility.  |  Bione, S., et al. 1998. Am J Hum Genet. 62: 533-41. PMID: 9497258
12. Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the yeast response regulator protein Skn7.  |  Alberts, AS., et al. 1998. J Biol Chem. 273: 8616-22. PMID: 9535835

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dia 1 Antibody (E-4) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Dia 1 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dia 1 Antibody (E-4) Specifications

Alternate Product Name: Dia 1

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1219-1248

Epitope Target: Dia 1

Epitope Species: human

Product Reactivity/Detection: Dia 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Dia 1 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin