DHS Antibody (C-7): sc-376580

DHS Antibody (C-7) is a mouse monoclonal IgG1 antibody that detects deoxyhypusine synthase (DHS) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DHS plays a pivotal role in the post-translational modification of eukaryotic initiation factor 5A (eIF-5A) by facilitating the formation of hypusine, a unique modification that is critical for eIF-5A′s function in promoting cell proliferation and survival. The importance of hypusine lies in its influence on the stability and activity of eIF-5A, which is essential for various cellular processes, including translation and cell cycle progression. By catalyzing the NAD-dependent oxidative cleavage of spermidine, DHS initiates the hypusine biosynthesis pathway, ultimately leading to the modification of eIF-5A that is necessary for biological activity. DHS (C-7) antibody serves as an invaluable tool for researchers studying the mechanisms of cell growth and the role of hypusine in cellular functions, providing insights into potential therapeutic targets for diseases characterized by dysregulated cell proliferation.

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DHS Antibody (C-7) References:

1. Identification of lysine350 of yeast deoxyhypusine synthase as the site of enzyme intermediate formation.  |  Wolff, EC. and Park, MH. 1999. Yeast. 15: 43-50. PMID: 10028184
2. Complex formation between deoxyhypusine synthase and its protein substrate, the eukaryotic translation initiation factor 5A (eIF5A) precursor.  |  Lee, YB., et al. 1999. Biochem J. 340 (Pt 1): 273-81. PMID: 10229683
3. Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase.  |  Ober, D. and Hartmann, T. 1999. Proc Natl Acad Sci U S A. 96: 14777-82. PMID: 10611289
4. Deoxyhypusine synthase generates and uses bound NADH in a transient hydride transfer mechanism.  |  Wolff, EC., et al. 2000. J Biol Chem. 275: 9170-7. PMID: 10734052
5. Molecular evolution by change of function. Alkaloid-specific homospermidine synthase retained all properties of deoxyhypusine synthase except binding the eIF5A precursor protein.  |  Ober, D., et al. 2003. J Biol Chem. 278: 12805-12. PMID: 12562768
6. Protein kinase CK2 phosphorylates and interacts with deoxyhypusine synthase in HeLa cells.  |  Kang, KR. and Chung, SI. 2003. Exp Mol Med. 35: 556-64. PMID: 14749535
7. Evaluation of deoxyhypusine synthase inhibitors targeting BCR-ABL positive leukemias.  |  Ziegler, P., et al. 2012. Invest New Drugs. 30: 2274-83. PMID: 22415796
8. Deoxyhypusine synthase (DHPS) inhibitor GC7 induces p21/Rb-mediated inhibition of tumor cell growth and DHPS expression correlates with poor prognosis in neuroblastoma patients.  |  Bandino, A., et al. 2014. Cell Oncol (Dordr). 37: 387-98. PMID: 25315710
9. Half Way to Hypusine-Structural Basis for Substrate Recognition by Human Deoxyhypusine Synthase.  |  Wątor, E., et al. 2020. Biomolecules. 10: PMID: 32235505
10. Structural characterization of the (deoxy)hypusination in Trichomonas vaginalis questions the bifunctionality of deoxyhypusine synthase.  |  Wątor, E., et al. 2024. FEBS J. 291: 3856-3869. PMID: 38923395

The preferred DHS antibody is DHS Antibody (A-10): sc-365077.