Anti-Dhh Antibody (D-4) recommended for detection of the N-terminal subunit of Dhh of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Dhh Antibody (D-4): sc-515757

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Datasheets

    • Dhh Antibody (D-4) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 29-51 at the N-terminus of Dhh of mouse origin
    • recommended for detection of the N-terminal subunit of Dhh of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Dhh Antibody (D-4) for WB applications. This reagent is now offered in a bundle with Dhh Antibody (D-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
loading

How much sample do you recommend loading on the gel for Western blot detection using Dhh (D-4): sc-515757?

Asked by: Dr Ninau Qelp
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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