DGK-θ Antibody (E-5): sc-137197
- DGK-θ Antibody (E-5) is a mouse monoclonal IgG2a κ DGK-θ antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 691-820 mapping near the C-terminus of DGK-θ of human origin
- DGK-theta Antibody (E-5) is recommended for detection of DGK-θ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DGK-theta Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for DGK-θ Antibody (E-5) for WB applications. This reagent is now offered in a bundle with DGK-θ Antibody (E-5) (see ordering information below).
DGK-θ Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects DGK-theta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DGK-θ (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DGK-theta protein plays a crucial role in cellular signaling by phosphorylating diacylglycerol (DAG) to generate phosphatidic acid, both of which are important lipid second messengers involved in various signaling pathways. This process is vital for regulating cell activation and secretion, influencing numerous physiological responses, including cell growth, differentiation, and survival. DGK-theta is predominantly expressed in the cerebellum and hippocampus, areas of the brain associated with motor control and memory, respectively. DGK-theta′s presence in these regions underscores its potential significance in neurophysiological processes and implications in neurological disorders. Through interactions with other diacylglycerol kinases and various signaling molecules, anti-DGK-theta antibody (E-5) serves as a valuable tool for researchers investigating the intricate roles of DGK-theta in cellular signaling and its impact on health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DGK-θ Antibody (E-5) References:
- Mammalian diacylglycerol kinases, a family of lipid kinases with signaling functions. | Topham, MK. and Prescott, SM. 1999. J Biol Chem. 274: 11447-50. PMID: 10206945
- Purification, cDNA-cloning and expression of human diacylglycerol kinase. | Schaap, D., et al. 1990. FEBS Lett. 275: 151-8. PMID: 2175712
- Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons. | Goto, K. and Kondo, H. 1993. Proc Natl Acad Sci U S A. 90: 7598-602. PMID: 7689223
- Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells. | Kai, M., et al. 1994. J Biol Chem. 269: 18492-8. PMID: 8034597
- Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats. | Masai, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 11157-61. PMID: 8248222
- Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein-tyrosine kinases. | Sakane, F., et al. 1996. J Biol Chem. 271: 8394-401. PMID: 8626538
- Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates. | Tang, W., et al. 1996. J Biol Chem. 271: 10237-41. PMID: 8626589
- Cloning and characterization of a glucocorticoid-induced diacylglycerol kinase. | Klauck, TM., et al. 1996. J Biol Chem. 271: 19781-8. PMID: 8702685
- The cloning and characterization of a novel human diacylglycerol kinase, DGKiota. | Ding, L., et al. 1998. J Biol Chem. 273: 32746-52. PMID: 9830018
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGK-θ Antibody (E-5) | sc-137197 | 200 µg/ml | $316.00 | |||
DGK-θ Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-525754 | 200 µg Ab; 10 µg BP | $354.00 | |||
DGK-θ Antibody (E-5) AC | sc-137197 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DGK-θ Antibody (E-5) HRP | sc-137197 HRP | 200 µg/ml | $316.00 | |||
DGK-θ Antibody (E-5) FITC | sc-137197 FITC | 200 µg/ml | $330.00 | |||
DGK-θ Antibody (E-5) PE | sc-137197 PE | 200 µg/ml | $343.00 | |||
DGK-θ Antibody (E-5) Alexa Fluor® 488 | sc-137197 AF488 | 200 µg/ml | $357.00 | |||
DGK-θ Antibody (E-5) Alexa Fluor® 546 | sc-137197 AF546 | 200 µg/ml | $357.00 | |||
DGK-θ Antibody (E-5) Alexa Fluor® 594 | sc-137197 AF594 | 200 µg/ml | $357.00 | |||
DGK-θ Antibody (E-5) Alexa Fluor® 647 | sc-137197 AF647 | 200 µg/ml | $357.00 | |||
DGK-θ Antibody (E-5) Alexa Fluor® 680 | sc-137197 AF680 | 200 µg/ml | $357.00 | |||
DGK-θ Antibody (E-5) Alexa Fluor® 790 | sc-137197 AF790 | 200 µg/ml | $357.00 |