Date published: 2026-4-24

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DGK-ι Antibody (D-4): sc-271383

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Datasheets
  • DGK-ι Antibody (D-4) is a mouse monoclonal IgM κ provided at 200 µg/ml
  • raised against amino acids 854-1019 mapping near the C-terminus of DGK-ι of human origin
  • recommended for detection of DGK-ι of human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DGK-ι Antibody (D-4). This work is in progress.
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    DGK-ι Antibody (D-4) is a mouse monoclonal IgM antibody that detects diacylglycerol kinase iota (DGK-ι) in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DGK-ι plays a crucial role in lipid signaling by phosphorylating diacylglycerol to generate phosphatidic acid, a key second messenger involved in numerous cellular processes such as cell proliferation, differentiation, and survival. DGK-ι (D-4) antibody helps researchers study signaling pathways that influence cell fate decisions, particularly in neuronal function and development, where DGK-ι is predominantly expressed in the brain and retina. This expression pattern suggests DGK-ι may be integral to the regulation of synaptic signaling and plasticity, which are vital for learning and memory. Additionally, DGK-ι is characterized by specific structural motifs that contribute to regulatory functions, allowing for the fine-tuning of lipid-mediated signaling cascades. With diverse applications and significant role in cellular signaling, anti-DGK-ι antibody (D-4) serves as an essential tool for investigating the complexities of lipid signaling in human health and disease.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    DGK-ι Antibody (D-4)

    sc-271383
    200 µg/ml
    $322.00

    What is the recommended fixation method for immunofluorescence staining with DGK-ι (D-4): sc-271383 monoclonal antibody?

    Asked by: Randy McDonald
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-02-25
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    Rated 5 out of 5 by from Great immunofluorescence cytoplasmic stainingGreat immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-03-18
    Rated 5 out of 5 by from Good Western Blot data of DGKGood Western Blot data of DGK- expression in HeLa whole cell lysate. -SCBT QC
    Date published: 2014-09-02
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    DGK-ι Antibody (D-4) is rated 5.0 out of 5 by 2.
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