DGK-β Antibody (G-11): sc-515090
- DGK-β Antibody (G-11) is a mouse monoclonal IgG2b κ DGK-β antibody provided at 200 µg/ml
- raised against amino acids 27-138 mapping near the N-terminus of DGK-β of human origin
- DGK-beta Antibody (G-11) is recommended for detection of DGK-β of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DGK-beta Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for DGK-β Antibody (G-11) for WB applications. This reagent is now offered in a bundle with DGK-β Antibody (G-11) (see ordering information below).
DGK-β Antibody (G-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects DGK-beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DGK-β (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The diacylglycerol kinase beta (DGK-β) protein plays a crucial role in cellular signaling by phosphorylating diacylglycerol (DAG) to generate phosphatidic acid, both of which are important lipid second messengers involved in various signaling pathways. Proper functioning of DGK-β is vital for regulating cell activation and secretion processes, which can influence cell survival and death. Notably, DGK-β is predominantly expressed in specific regions of the brain, such as the caudate putamen and olfactory tubercle, highlighting potential significance in neurobiological functions and disorders. DGK-β presence in these areas suggests involvement in modulating neuronal signaling and plasticity, making DGK-β a valuable target for research into neurological diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DGK-β Antibody (G-11) References:
- Mammalian diacylglycerol kinases, a family of lipid kinases with signaling functions. | Topham, MK. and Prescott, SM. 1999. J Biol Chem. 274: 11447-50. PMID: 10206945
- Purification, cDNA-cloning and expression of human diacylglycerol kinase. | Schaap, D., et al. 1990. FEBS Lett. 275: 151-8. PMID: 2175712
- Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons. | Goto, K. and Kondo, H. 1993. Proc Natl Acad Sci U S A. 90: 7598-602. PMID: 7689223
- Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells. | Kai, M., et al. 1994. J Biol Chem. 269: 18492-8. PMID: 8034597
- Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats. | Masai, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 11157-61. PMID: 8248222
- Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein-tyrosine kinases. | Sakane, F., et al. 1996. J Biol Chem. 271: 8394-401. PMID: 8626538
- Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates. | Tang, W., et al. 1996. J Biol Chem. 271: 10237-41. PMID: 8626589
- Cloning and characterization of a glucocorticoid-induced diacylglycerol kinase. | Klauck, TM., et al. 1996. J Biol Chem. 271: 19781-8. PMID: 8702685
- The cloning and characterization of a novel human diacylglycerol kinase, DGKiota. | Ding, L., et al. 1998. J Biol Chem. 273: 32746-52. PMID: 9830018
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGK-β Antibody (G-11) | sc-515090 | 200 µg/ml | $316.00 | |||
DGK-β Antibody (G-11): m-IgG2b BP-HRP Bundle | sc-548810 | 200 µg Ab; 10 µg BP | $354.00 | |||
DGK-β Antibody (G-11) AC | sc-515090 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DGK-β Antibody (G-11) HRP | sc-515090 HRP | 200 µg/ml | $316.00 | |||
DGK-β Antibody (G-11) FITC | sc-515090 FITC | 200 µg/ml | $330.00 | |||
DGK-β Antibody (G-11) PE | sc-515090 PE | 200 µg/ml | $343.00 | |||
DGK-β Antibody (G-11) Alexa Fluor® 488 | sc-515090 AF488 | 200 µg/ml | $357.00 | |||
DGK-β Antibody (G-11) Alexa Fluor® 546 | sc-515090 AF546 | 200 µg/ml | $357.00 | |||
DGK-β Antibody (G-11) Alexa Fluor® 594 | sc-515090 AF594 | 200 µg/ml | $357.00 | |||
DGK-β Antibody (G-11) Alexa Fluor® 647 | sc-515090 AF647 | 200 µg/ml | $357.00 | |||
DGK-β Antibody (G-11) Alexa Fluor® 680 | sc-515090 AF680 | 200 µg/ml | $357.00 | |||
DGK-β Antibody (G-11) Alexa Fluor® 790 | sc-515090 AF790 | 200 µg/ml | $357.00 |