demethylated-PP2A-C Antibody (4B7): sc-13601

demethylated-PP2A-C Antibody (4B7) is a mouse monoclonal IgG1 kappa light chain antibody that detects demethylated-PP2A-C protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin embedded sections (IHCP). demethylated-PP2A-C (4B7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. demethylated-PP2A-C protein plays a crucial role in cellular signaling pathways, particularly in the regulation of cell growth and division, as demethylated-PP2A-C is a key component of the protein phosphatase 2A (PP2A) complex. This complex is essential for dephosphorylating serine and threonine residues on target proteins, thereby modulating their activity and function. PP2A maintains cellular homeostasis and prevents uncontrolled cell proliferation, which is a hallmark of cancer. Additionally, PP2A catalytic subunit interacts with various regulatory subunits, such as PP2A-Aα, PP2A-Bα, and others, which dictate the substrate specificity and localization of the phosphatase, further underscoring the complexity and significance of PP2A in cellular processes.

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demethylated-PP2A-C Antibody (4B7) References:

1. Methylated C-terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory Balpha subunit.  |  Bryant, JC., et al. 1999. Biochem J. 339 (Pt 2): 241-6. PMID: 10191253
2. A positive role for the PP2A catalytic subunit in Wnt signal transduction.  |  Ratcliffe, MJ., et al. 2000. J Biol Chem. 275: 35680-3. PMID: 11007767
3. Methylation of the C-terminal leucine residue of the PP2A catalytic subunit is unnecessary for the catalytic activity and the binding of regulatory subunit (PR55/B).  |  Ikehara, T., et al. 2007. Biochem Biophys Res Commun. 354: 1052-7. PMID: 17274953
4. (DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit.  |  Wang, X., et al. 2017. Oncotarget. 8: 33197-33213. PMID: 28402257
5. PP2A catalytic subunit silence by microRNA-429 activates AMPK and protects osteoblastic cells from dexamethasone.  |  Guo, S., et al. 2017. Biochem Biophys Res Commun. 487: 660-665. PMID: 28438603
6. Activation of the PP2A catalytic subunit by ivabradine attenuates the development of diabetic cardiomyopathy.  |  Zuo, GF., et al. 2019. J Mol Cell Cardiol. 130: 170-183. PMID: 30998977
7. Antibodies recognizing the C terminus of PP2A catalytic subunit are unsuitable for evaluating PP2A activity and holoenzyme composition.  |  Frohner, IE., et al. 2020. Sci Signal. 13: PMID: 31992581
8. PP2A-C may be a promising candidate for postmortem interval estimation.  |  Wang, J., et al. 2021. Int J Legal Med. 135: 837-844. PMID: 33409557
9. PP2A Catalytic Subunit α promotes fibroblast activation and kidney fibrosis via ERK pathway.  |  Lu, Q., et al. 2022. Cell Signal. 90: 110187. PMID: 34780974
10. PP2A catalytic subunit alpha is critically required for CD8+ T-cell homeostasis and antibacterial responses.  |  Zhou, X., et al. 2024. Eur J Immunol. 54: e2451080. PMID: 39072720

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-demethylated-PP2A-C Antibody (4B7) has 36 citations in a variety of scientific publications.

Primary antibodies like Anti-demethylated-PP2A-C Antibody (4B7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

demethylated-PP2A-C Antibody (4B7) Specifications

Alternate Product Name: demethylated PP2A phosphatase catalytic subunit C

Product Clone Name: 4B7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: demethylated-PP2A-C

Epitope Species: mouse

Product Reactivity/Detection: demethylated-PP2A-C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Drosophila, Xenopus, S. pombe

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 36 citations in various scientific publications

Available Conjugates/Forms for demethylated-PP2A-C Antibody (4B7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin