Deltex-3 Antibody (C-10): sc-376439
- Deltex-3 Antibody (C-10) is a mouse monoclonal IgG1 κ Deltex-3 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-140 mapping at the N-terminus of Deltex-3 of human origin
- Deltex-3 Antibody (C-10) is recommended for detection of Deltex-3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Deltex-3 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Deltex-3 Antibody (C-10) for WB applications. These reagents are now offered in bundles with Deltex-3 Antibody (C-10) (see ordering information below).
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Deltex-3 Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Deltex-3 (DTX3) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DTX3 antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Deltex family of proteins, which includes DTX1, DTX2, DTX3, and DTX4, are mammalian homologs of the Drosophila Deltex protein and are characterized by the presence of two WWE domains and a C-terminal RING-finger domain, which are critical for their function as E3 ubiquitin ligases. DTX3, also known as RNF154 (RING finger protein 154), is a 347 amino acid cytoplasmic protein that plays a dual role as both a negative and positive regulator of the Notch signaling pathway, depending on the specific developmental and cellular context. DTX3 primarily functions as a homomultimer but can also form heteromultimers with other Deltex family members, enhancing regulatory capabilities. DTX3 exhibits highest E3 ligase activity in conjunction with the E2 enzyme UBE2D, underscoring the importance of protein-protein interactions in functional regulation. Additionally, two isoforms of DTX3 arise from alternative splicing, contributing to the complexity of regulatory roles in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Deltex-3 Antibody (C-10) References:
- The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity. | Takeyama, K., et al. 2003. J Biol Chem. 278: 21930-7. PMID: 12670957
- A new niche for Notch on Deltex? | Blacklow, SC. 2005. Structure. 13: 1579-80. PMID: 16271878
- T cells develop normally in the absence of both Deltex1 and Deltex2. | Lehar, SM. and Bevan, MJ. 2006. Mol Cell Biol. 26: 7358-71. PMID: 16923970
- Itch/AIP4 mediates Deltex degradation through the formation of K29-linked polyubiquitin chains. | Chastagner, P., et al. 2006. EMBO Rep. 7: 1147-53. PMID: 17028573
- Specificity and autoregulation of Notch binding by tandem WW domains in suppressor of Deltex. | Jennings, MD., et al. 2007. J Biol Chem. 282: 29032-29042. PMID: 17656366
- Drosophila HOPS and AP-3 complex genes are required for a Deltex-regulated activation of notch in the endosomal trafficking pathway. | Wilkin, M., et al. 2008. Dev Cell. 15: 762-72. PMID: 19000840
- Regulation of NOTCH signaling by reciprocal inhibition of HES1 and Deltex 1 and its role in osteosarcoma invasiveness. | Zhang, P., et al. 2010. Oncogene. 29: 2916-26. PMID: 20208568
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Deltex-3 Antibody (C-10) | sc-376439 | 200 µg/ml | $316.00 | |||
Deltex-3 Antibody (C-10): m-IgG Fc BP-HRP Bundle | sc-529869 | 200 µg Ab; 10 µg BP | $354.00 | |||
Deltex-3 Antibody (C-10): m-IgGκ BP-HRP Bundle | sc-522956 | 200 µg Ab, 40 µg BP | $354.00 | |||
Deltex-3 Antibody (C-10) AC | sc-376439 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Deltex-3 Antibody (C-10) HRP | sc-376439 HRP | 200 µg/ml | $316.00 | |||
Deltex-3 Antibody (C-10) FITC | sc-376439 FITC | 200 µg/ml | $330.00 | |||
Deltex-3 Antibody (C-10) PE | sc-376439 PE | 200 µg/ml | $343.00 | |||
Deltex-3 Antibody (C-10) Alexa Fluor® 488 | sc-376439 AF488 | 200 µg/ml | $357.00 | |||
Deltex-3 Antibody (C-10) Alexa Fluor® 546 | sc-376439 AF546 | 200 µg/ml | $357.00 | |||
Deltex-3 Antibody (C-10) Alexa Fluor® 594 | sc-376439 AF594 | 200 µg/ml | $357.00 | |||
Deltex-3 Antibody (C-10) Alexa Fluor® 647 | sc-376439 AF647 | 200 µg/ml | $357.00 | |||
Deltex-3 Antibody (C-10) Alexa Fluor® 680 | sc-376439 AF680 | 200 µg/ml | $357.00 | |||
Deltex-3 Antibody (C-10) Alexa Fluor® 790 | sc-376439 AF790 | 200 µg/ml | $357.00 |