Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Delta CRISPR/Cas9 KO Plasmid (h): sc-400790
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Delta CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Delta genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Delta Antibody (G-1): sc-377310
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Delta CRISPR/Cas9 KO Plasmid (h) | sc-400790 | 20 µg | $397.00 |
Overview
DLL1 encodes the human Delta-like 1 (Delta) ligand, a membrane-bound activator of Notch receptors that mediates juxtacrine signaling to control cell fate decisions, tissue patterning, and stem/progenitor maintenance. DLL1–NOTCH engagement triggers proteolytic release of the Notch intracellular domain and transcriptional programs via RBPJ/CSL that influence differentiation, proliferation, and apoptosis. This pathway intersects with developmental programs and microenvironmental cues, and its dysregulation is frequently studied in contexts such as tumor biology, neurogenesis, and immune cell development. DLL1 expression and signaling dynamics are also relevant to epithelial–mesenchymal transitions and angiogenic regulation through coordinated Notch ligand–receptor balance.
Delta CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DLL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DLL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DLL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Delta protein expression.
This CRISPR knockout system enables efficient generation of DLL1-deficient cell models for investigation of Delta signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DLL1 exon(s) critical for Delta function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DLL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Delta CRISPR/Cas9 KO Plasmid (h) and Delta CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DLL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Delta HDR Plasmid (h) and Delta HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DLL1 homology arms to support homology-directed repair at defined DLL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.