Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DEF6 CRISPR/Cas9 KO Plasmid (h): sc-405524
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DEF6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DEF6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DEF6 Antibody (SW-9): sc-81992
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DEF6 CRISPR/Cas9 KO Plasmid (h) | sc-405524 | 20 µg | $397.00 |
Overview
DEF6 (differentially expressed in FDCP 6 homolog) encodes an immune-associated guanine nucleotide exchange factor that coordinates signaling downstream of antigen receptors, integrating membrane proximal events with actin remodeling and transcriptional responses. In lymphocytes, DEF6 modulates small GTPase pathways and contributes to Ca²⁺-dependent signaling, influencing NFAT-driven gene expression and cytokine programs that shape activation and differentiation. Through these functions, DEF6 participates in immunological synapse organization and broader regulation of adaptive immune homeostasis. Dysregulated DEF6 activity or expression has been linked to aberrant immune signaling and susceptibility to immune-mediated pathology, making it a useful target for mechanistic studies of immune regulation.
DEF6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DEF6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DEF6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DEF6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DEF6 protein expression.
This CRISPR knockout system enables efficient generation of DEF6-deficient cell models for investigation of DEF6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DEF6 exon(s) critical for DEF6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DEF6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DEF6 CRISPR/Cas9 KO Plasmid (h) and DEF6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DEF6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DEF6 HDR Plasmid (h) and DEF6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DEF6 homology arms to support homology-directed repair at defined DEF6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.