Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DEC-205 CRISPR/Cas9 KO Plasmid (h): sc-417220
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DEC-205 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DEC-205 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DEC-205 Antibody (F-4): sc-515016
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DEC-205 CRISPR/Cas9 KO Plasmid (h) | sc-417220 | 20 µg | $397.00 |
Overview
LY75 encodes DEC-205 (CD205), a multilectin endocytic receptor of the mannose receptor family that is highly expressed by dendritic cells and other antigen-presenting cells. DEC-205 promotes high-efficiency uptake and routing of protein antigens into endosomal/lysosomal compartments, supporting MHC class II presentation and, under some conditions, cross-presentation for CD8+ T cell priming. Through its role in antigen capture, processing, and immune tolerance versus activation, LY75 is frequently used to interrogate dendritic-cell maturation programs and adaptive immune polarization. Altered DEC-205 expression and trafficking have been associated with inflammatory and autoimmune contexts and are also studied in tumor immunology as markers and functional mediators of antigen presentation states.
DEC-205 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LY75 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LY75 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LY75 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DEC-205 protein expression.
This CRISPR knockout system enables efficient generation of LY75-deficient cell models for investigation of DEC-205 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LY75 exon(s) critical for DEC-205 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LY75 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DEC-205 CRISPR/Cas9 KO Plasmid (h) and DEC-205 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LY75 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DEC-205 HDR Plasmid (h) and DEC-205 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LY75 homology arms to support homology-directed repair at defined LY75 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.