DEC-205 Antibody (F-4): sc-515016

DEC-205 Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects DEC-205 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-DEC-205 antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DEC-205, also known as LY75 or lymphocyte antigen 75, is a 1,695-residue multilectin receptor that plays a crucial role in the immune system by mediating the uptake of antigens and their presentation to T cells. DEC-205 is primarily expressed in mature dendritic cells, where DEC-205 facilitates the targeting of membrane receptors to endosomes or lysosomes that are rich in major histocompatibility complex class II (MHC II) products, thereby enhancing the immune response. The structure of DEC-205 includes an extracellular N-terminal cysteine-rich domain, a fibronectin type II domain, ten C-type carbohydrate recognition domains, a single transmembrane region, and a small cytoplasmic C-terminal domain that contains a critical tyrosine residue at position 1679. This structural configuration is essential for DEC-205 function, as DEC-205 can elicit either an agonistic or antagonistic effect on interleukin-4 (IL-4) signaling, influencing the maturation of epithelial cells and the proliferation of T cells. DEC-205 monoclonal antibody (F-4) versatility in various applications makes DEC-205 monoclonal antibody (F-4) an invaluable tool for researchers studying immune responses and dendritic cell biology.

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DEC-205 Antibody (F-4) References:

1. DEC-205 as a marker of dendritic cells with regulatory effects on CD8 T cell responses.  |  Kronin, V., et al. 2000. Int Immunol. 12: 731-5. PMID: 10784619
2. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells.  |  Guo, M., et al. 2000. Hum Immunol. 61: 729-38. PMID: 10980384
3. The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments.  |  Mahnke, K., et al. 2000. J Cell Biol. 151: 673-84. PMID: 11062267
4. Vaccination with filamentous bacteriophages targeting DEC-205 induces DC maturation and potent anti-tumor T-cell responses in the absence of adjuvants.  |  Sartorius, R., et al. 2011. Eur J Immunol. 41: 2573-84. PMID: 21688262
5. DEC-205-mediated antigen targeting to steady-state dendritic cells induces deletion of diabetogenic CD8⁺ T cells independently of PD-1 and PD-L1.  |  Mukherjee, G., et al. 2013. Int Immunol. 25: 651-60. PMID: 24021877
6. Triggering positive selection of germinal center B cells by antigen targeting to DEC-205.  |  Pasqual, G., et al. 2015. Methods Mol Biol. 1291: 125-34. PMID: 25836306
7. Surface display of an anti-DEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivo.  |  Michon, C., et al. 2015. Microb Cell Fact. 14: 95. PMID: 26141059
8. Stimulation of Innate and Adaptive Immunity by Using Filamentous Bacteriophage fd Targeted to DEC-205.  |  D'Apice, L., et al. 2015. J Immunol Res. 2015: 585078. PMID: 26380324
9. The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing.  |  Jiang, W., et al. 1995. Nature. 375: 151-5. PMID: 7753172
10. Bladder carcinomas and normal urothelium universally express gp200-MR6, a molecule functionally associated with the interleukin 4 receptor (CD 124).  |  Tungekar, MF., et al. 1996. Br J Cancer. 73: 429-32. PMID: 8595155
11. cDNA cloning of human DEC-205, a putative antigen-uptake receptor on dendritic cells.  |  Kato, M., et al. 1998. Immunogenetics. 47: 442-50. PMID: 9553150
12. The gp200-MR6 molecule which is functionally associated with the IL-4 receptor modulates B cell phenotype and is a novel member of the human macrophage mannose receptor family.  |  McKay, PF., et al. 1998. Eur J Immunol. 28: 4071-83. PMID: 9862343

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DEC-205 Antibody (F-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-DEC-205 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DEC-205 Antibody (F-4) Specifications

Alternate Product Name: DEC-205

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1473-1722

Epitope Target: DEC-205

Epitope Species: human

Product Reactivity/Detection: precursor and mature forms of DEC-205 and DEC-205/DCL-1 fusion protein

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for DEC-205 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin