DDX32 Antibody (H-3): sc-398743
- DDX32 Antibody (H-3) is a mouse monoclonal IgG2a κ DDX32 antibody provided at 200 µg/ml
- raised against amino acids 184-287 mapping within an internal region of DDX32 of human origin
- DDX32 Antibody (H-3) is recommended for detection of DDX32 of human origin by WB, IP, IF and ELISA
- Anti-DDX32 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX32 Antibody (H-3) for WB applications. These reagents are now offered in bundles with DDX32 Antibody (H-3) (see ordering information below).
DDX32 Antibody (H-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects DDX32 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDX32 monoclonal antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDX32, also known as DEAH box polypeptide 32 or DHLP1, is a 743 amino acid nuclear protein that plays a crucial role in RNA metabolism and is a member of the DEAD box helicase family, characterized by the conserved Asp-Glu-Ala-Asp motif. DDX32 is primarily located in the nucleus and mitochondria, where DDX32 is involved in unwinding RNA structures, essential for processes such as ribosome and spliceosome assembly. Proper functioning of DDX32 is vital for cellular processes, including embryogenesis and cellular growth, as DDX32 helps regulate RNA secondary structures that can influence gene expression and cellular responses. Notably, DDX32 is up-regulated by ionomycin in T lymphocytes, indicating a potential role in immune responses, and DDX32 expression is altered in conditions such as acute lymphoblastic leukemia, suggesting involvement in cancer biology. Given DDX32′s significance in RNA processing and cellular regulation, DDX32 may also play a pivotal role in colorectal cancer development and in modulating T-cell responses to apoptotic signals, making DDX32 a valuable target for research in cancer and immunology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDX32 Antibody (H-3) References:
- The novel helicase homologue DDX32 is down-regulated in acute lymphoblastic leukemia. | Abdelhaleem, M. 2002. Leuk Res. 26: 945-54. PMID: 12163057
- DHX32 expression suggests a role in lymphocyte differentiation. | Abdelhaleem, M., et al. 2005. Anticancer Res. 25: 2645-8. PMID: 16080506
- Expression of DHX32 in lymphoid tissues. | Alli, Z., et al. 2005. Exp Mol Pathol. 79: 219-23. PMID: 16181624
- The activation-induced expression of DHX32 in Jurkat T cells is specific and involves calcium and nuclear factor of activated T cells. | Alli, Z., et al. 2005. Cell Immunol. 237: 141-6. PMID: 16414036
- Nuclear and mitochondrial localization of the putative RNA helicase DHX32. | Alli, Z., et al. 2006. Exp Mol Pathol. 81: 245-8. PMID: 16959245
- Altered distribution of heat shock protein 60 (Hsp60) with dysregulated expression of DHX32. | Chen, Y., et al. 2007. Exp Mol Pathol. 82: 256-61. PMID: 17174952
- Characterization of murine Dhx32. | Alli, Z., et al. 2007. Exp Mol Pathol. 83: 115-8. PMID: 17239371
- A role for DHX32 in regulating T-cell apoptosis. | Alli, Z., et al. 2007. Anticancer Res. 27: 373-7. PMID: 17352256
- Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer. | Huang, C., et al. 2009. J Exp Clin Cancer Res. 28: 11. PMID: 19161603
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX32 Antibody (H-3) | sc-398743 | 200 µg/ml | $322.00 | |||
DDX32 Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-530913 | 200 µg Ab; 10 µg BP | $361.00 | |||
DDX32 Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-524467 | 200 µg Ab, 40 µg BP | $361.00 | |||
DDX32 Antibody (H-3): m-IgG2a BP-HRP Bundle | sc-547627 | 200 µg Ab; 10 µg BP | $361.00 | |||
DDX32 Antibody (H-3) AC | sc-398743 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DDX32 Antibody (H-3) HRP | sc-398743 HRP | 200 µg/ml | $322.00 | |||
DDX32 Antibody (H-3) FITC | sc-398743 FITC | 200 µg/ml | $336.00 | |||
DDX32 Antibody (H-3) PE | sc-398743 PE | 200 µg/ml | $349.00 | |||
DDX32 Antibody (H-3) Alexa Fluor® 488 | sc-398743 AF488 | 200 µg/ml | $364.00 | |||
DDX32 Antibody (H-3) Alexa Fluor® 546 | sc-398743 AF546 | 200 µg/ml | $364.00 | |||
DDX32 Antibody (H-3) Alexa Fluor® 594 | sc-398743 AF594 | 200 µg/ml | $364.00 | |||
DDX32 Antibody (H-3) Alexa Fluor® 647 | sc-398743 AF647 | 200 µg/ml | $364.00 | |||
DDX32 Antibody (H-3) Alexa Fluor® 680 | sc-398743 AF680 | 200 µg/ml | $364.00 | |||
DDX32 Antibody (H-3) Alexa Fluor® 790 | sc-398743 AF790 | 200 µg/ml | $364.00 |