DDX25 Antibody (G-4): sc-166289

DDX25 Antibody (G-4) is a mouse monoclonal IgG1 antibody that detects DDX25 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDX25, also known as gonadotropin-regulated testicular RNA helicase, is a testis-specific member of the DEAD-box protein family, predominantly expressed in Leydig and germ cells. DDX25 plays a crucial role in spermatogenesis, possessing both ATPase and RNA helicase activities, which are essential for regulating translational processes, including mRNA nuclear export during spermatid development. DDX25 undergoes phosphorylation on threonine residues, with the phosphorylated form localized exclusively in the cytoplasm, highlighting dynamic regulation and functional significance in cellular processes. DDX25′s importance extends to germ cell development, where DDX25 is up-regulated by gonadotropin at the transcriptional level, and genetic variations in DDX25 have been implicated in male infertility due to spermatogenic impairment. Anti-DDX25 antibody (G-4) serves as an invaluable tool for researchers studying reproductive biology and related fields.

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DDX25 Antibody (G-4) References:

1. Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is essential for spermatid development and completion of spermatogenesis.  |  Tsai-Morris, CH., et al. 2004. Proc Natl Acad Sci U S A. 101: 6373-8. PMID: 15096601
2. Gonadotropin-regulated testicular helicase (GRTH/DDX25): an essential regulator of spermatogenesis.  |  Dufau, ML. and Tsai-Morris, CH. 2007. Trends Endocrinol Metab. 18: 314-20. PMID: 17889551
3. Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25): a multifunctional protein essential for spermatogenesis.  |  Tsai-Morris, CH., et al. 2010. J Androl. 31: 45-52. PMID: 19875492
4. Expression of DDX25 in nuage components of mammalian spermatogenic cells: immunofluorescence and immunoelectron microscopic study.  |  Onohara, Y. and Yokota, S. 2012. Histochem Cell Biol. 137: 37-51. PMID: 22038044
5. Role of gonadotropin regulated testicular RNA helicase (GRTH/Ddx25) on polysomal associated mRNAs in mouse testis.  |  Tsai-Morris, CH., et al. 2012. PLoS One. 7: e32470. PMID: 22479328
6. Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.  |  Han, Y., et al. 2019. Environ Pollut. 253: 538-551. PMID: 31330346
7. Linking Phospho-Gonadotropin Regulated Testicular RNA Helicase (GRTH/DDX25) to Histone Ubiquitination and Acetylation Essential for Spermatid Development During Spermiogenesis.  |  Kavarthapu, R., et al. 2020. Front Cell Dev Biol. 8: 310. PMID: 32478068
8. Role of Phosphorylated Gonadotropin-Regulated Testicular RNA Helicase (GRTH/DDX25) in the Regulation of Germ Cell Specific mRNAs in Chromatoid Bodies During Spermatogenesis.  |  Anbazhagan, R., et al. 2020. Front Cell Dev Biol. 8: 580019. PMID: 33425888
9. Blockade of GRTH/DDX25 Phosphorylation by Cyclic Peptides Provides an Avenue for Developing a Nonhormonal Male Contraceptive.  |  Raju, M., et al. 2021. J Med Chem. 64: 14715-14727. PMID: 34601876
10. Whole-exome sequencing improves the diagnosis and care of men with non-obstructive azoospermia.  |  Kherraf, ZE., et al. 2022. Am J Hum Genet. 109: 508-517. PMID: 35172124

The preferred DDX25 antibody is DDX25 Antibody (F-10): sc-271730.