DDX21 CRISPR/Cas9 KO Plasmid (h2): sc-405770-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX21 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDX21 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDX21 Antibody (D-8): sc-376953
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX21 CRISPR/Cas9 KO Plasmid (h2) | sc-405770-KO-2 | 20 µg | $397.00 |
Overview
DDX21 is a nucleolar DEAD-box RNA helicase that coordinates rRNA processing, ribosome biogenesis, and broader RNA metabolism through remodeling of RNA–protein complexes. It participates in nucleolar stress responses and interfaces with transcriptional programs that couple growth cues to protein synthesis capacity, influencing cell-cycle progression and proteostasis. DDX21 has also been implicated in innate immune signaling by modulating RNA-dependent pathways and the expression of interferon-stimulated genes. Dysregulated DDX21 function and altered ribosome biogenesis are frequently studied in the context of oncogenic transformation, genomic stress, and viral infection biology.
DDX21 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the DDX21 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DDX21 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DDX21 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDX21 protein expression.
This CRISPR knockout system enables efficient generation of DDX21-deficient cell models for investigation of DDX21 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DDX21 exon(s) critical for DDX21 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DDX21 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDX21 CRISPR/Cas9 KO Plasmid (h) and DDX21 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DDX21 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDX21 HDR Plasmid (h) and DDX21 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DDX21 homology arms to support homology-directed repair at defined DDX21 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.