DDX15 Antibody (E-6) recommended for detection of DDX15 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

DDX15 Antibody (E-6): sc-271686

DDX15 Antibody (E-6) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-DDX15 Antibody (E-6) is a mouse monoclonal IgG2a κ DDX15 antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 626-741 mapping near the C-terminus of DDX15 of human origin
    • Anti-DDX15 Antibody (E-6) is recommended for detection of DDX15 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-DDX15 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DDX15 (E-6): sc-271686.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for DDX15 Antibody (E-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with DDX15 Antibody (E-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I am using DDX15 (E-6): sc-271686 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good AB This antibody works pretty well for WB with strong signal.
Date published: 2017-07-06
Rated 5 out of 5 by from Recommended for WB Nice, strong and relatively clean bands in WB on K-562, NTERA-2 cl.D1, Daudi and NIH/3T3 whole cell lysates.
Date published: 2017-01-30
Rated 5 out of 5 by from Great immunoperoxidase nuclear staining in Great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from Good Western Blot data of DDX15 expression in MOLT Good Western Blot data of DDX15 expression in MOLT-4 and K-562 nuclear extracts. -SCBT QC
Date published: 2013-01-23
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