DDX1 Antibody (A-7): sc-271438
- DDX1 Antibody (A-7) is a mouse monoclonal IgG2a κ DDX1 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of DDX1 of human origin
- DDX1 Antibody (A-7) is recommended for detection of DDX1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DDX1 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-271438 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX1 Antibody (A-7) for WB applications. These reagents are now offered in bundles with DDX1 Antibody (A-7) (see ordering information below).
DDX1 Antibody (A-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects DDX1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDX1 Antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDX1 protein, a member of the DEAD box family of putative RNA helicases, plays a crucial role in regulating RNA metabolism, including processes such as translation initiation, splicing, and ribosome assembly. DDX1 function is vital for maintaining cellular homeostasis and facilitating proper gene expression, which is essential for processes like embryogenesis and cellular growth. DDX1 mRNA exhibits widespread distribution in human fetal tissues, indicating importance during development, while expression levels vary across different tissues, suggesting a regulatory role in tissue maturation. In chicken, DDX1 shares 93% identity with human counterpart and is predominantly expressed during early developmental stages, highlighting evolutionary conservation and functional significance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDX1 Antibody (A-7) References:
- Association of human DEAD box protein DDX1 with a cleavage stimulation factor involved in 3'-end processing of pre-MRNA. | Bléoo, S., et al. 2001. Mol Biol Cell. 12: 3046-59. PMID: 11598190
- Quantification of MYCN, DDX1, and NAG gene copy number in neuroblastoma using a real-time quantitative PCR assay. | De Preter, K., et al. 2002. Mod Pathol. 15: 159-66. PMID: 11850545
- Cloning and expression analysis of the chicken DEAD box gene DDX1. | Godbout, R., et al. 2002. Biochim Biophys Acta. 1574: 63-71. PMID: 11955614
- An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K. | Chen, HC., et al. 2002. J Biol Chem. 277: 40403-9. PMID: 12183465
- Alterations in the expression of DEAD-box and other RNA binding proteins during HIV-1 replication. | Krishnan, V. and Zeichner, SL. 2004. Retrovirology. 1: 42. PMID: 15588285
- No evidence for correlation of DDX1 gene amplification with improved survival probability in patients with MYCN-amplified neuroblastomas. | De Preter, K., et al. 2005. J Clin Oncol. 23: 3167-8; author reply 3168-70. PMID: 15860893
- The RNA helicase DDX1 is involved in restricted HIV-1 Rev function in human astrocytes. | Fang, J., et al. 2005. Virology. 336: 299-307. PMID: 15892970
- Dynamic nature of cleavage bodies and their spatial relationship to DDX1 bodies, Cajal bodies, and gems. | Li, L., et al. 2006. Mol Biol Cell. 17: 1126-40. PMID: 16371507
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX1 Antibody (A-7) | sc-271438 | 200 µg/ml | $322.00 | |||
DDX1 Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-529185 | 200 µg Ab; 10 µg BP | $361.00 | |||
DDX1 Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-521945 | 200 µg Ab, 40 µg BP | $361.00 | |||
DDX1 Antibody (A-7) X | sc-271438 X | 200 µg/0.1 ml | $322.00 | |||
DDX1 Antibody (A-7) AC | sc-271438 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DDX1 Antibody (A-7) HRP | sc-271438 HRP | 200 µg/ml | $322.00 | |||
DDX1 Antibody (A-7) FITC | sc-271438 FITC | 200 µg/ml | $336.00 | |||
DDX1 Antibody (A-7) PE | sc-271438 PE | 200 µg/ml | $349.00 | |||
DDX1 Antibody (A-7) Alexa Fluor® 488 | sc-271438 AF488 | 200 µg/ml | $364.00 | |||
DDX1 Antibody (A-7) Alexa Fluor® 546 | sc-271438 AF546 | 200 µg/ml | $364.00 | |||
DDX1 Antibody (A-7) Alexa Fluor® 594 | sc-271438 AF594 | 200 µg/ml | $364.00 | |||
DDX1 Antibody (A-7) Alexa Fluor® 647 | sc-271438 AF647 | 200 µg/ml | $364.00 | |||
DDX1 Antibody (A-7) Alexa Fluor® 680 | sc-271438 AF680 | 200 µg/ml | $364.00 | |||
DDX1 Antibody (A-7) Alexa Fluor® 790 | sc-271438 AF790 | 200 µg/ml | $364.00 |