DDEFL1 Antibody (C-3): sc-514132
- DDEFL1 Antibody (C-3) is a mouse monoclonal IgG1 κ DDEFL1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 872-892 at the C-terminus of DDEFL1 of human origin
- DDEFL1 Antibody (C-3) is recommended for detection of DDEFL1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DDEFL1 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DDEFL1 Antibody (C-3) for WB applications. These reagents are now offered in bundles with DDEFL1 Antibody (C-3) (see ordering information below).
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DDEFL1 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects DDEFL1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDEFL1 (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDEFL1, also known as development and differentiation enhancing factor-like 1, ACAP4, UPLC1, CENTB6, or ASAP3 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 3), is a 903 amino acid cytoplasmic protein that plays a crucial role in cellular processes. DDEFL1 belongs to the subfamily of ADP-ribosylation factor (Arf) GTPase-activating proteins and is characterized by the presence of two ankyrin repeats, an Arf-GAP domain, and a PH domain. DDEFL1 is primarily expressed in the lung, liver, blood leukocytes, and primary hepatocarcinoma, where DDEFL1 catalyzes the hydrolysis of GTP bound to Arf proteins, thereby promoting cell differentiation and migration. DDEFL1′s function is underscored by involvement in the pathogenesis of hepatocellular carcinoma, highlighting potential as a therapeutic target. Additionally, DDEFL1 exists in two isoforms produced by alternative splicing events and is encoded by a gene located on human chromosome 1, which spans 260 million base pairs and contains over 3,000 genes, comprising nearly 8% of the human genome.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDEFL1 Antibody (C-3) References:
- ACAPs are arf6 GTPase-activating proteins that function in the cell periphery. | Jackson, TR., et al. 2000. J Cell Biol. 151: 627-38. PMID: 11062263
- Molecular aspects of the cellular activities of ADP-ribosylation factors. | Randazzo, PA., et al. 2000. Sci STKE. 2000: re1. PMID: 11752622
- Isolation of development and differentiation enhancing factor-like 1 (DDEFL1) as a drug target for hepatocellular carcinomas. | Okabe, H., et al. 2004. Int J Oncol. 24: 43-8. PMID: 14654939
- Arf GAPs: multifunctional proteins that regulate membrane traffic and actin remodelling. | Randazzo, PA. and Hirsch, DS. 2004. Cell Signal. 16: 401-13. PMID: 14709330
- Proteomic identification and functional characterization of a novel ARF6 GTPase-activating protein, ACAP4. | Fang, Z., et al. 2006. Mol Cell Proteomics. 5: 1437-49. PMID: 16737952
- ArfGAP family proteins in cell adhesion, migration and tumor invasion. | Sabe, H., et al. 2006. Curr Opin Cell Biol. 18: 558-64. PMID: 16904307
- Arf GAPs and their interacting proteins. | Inoue, H. and Randazzo, PA. 2007. Traffic. 8: 1465-75. PMID: 17666108
- Arf GAPs as regulators of the actin cytoskeleton. | Randazzo, PA., et al. 2007. Biol Cell. 99: 583-600. PMID: 17868031
- ASAP3 is a focal adhesion-associated Arf GAP that functions in cell migration and invasion. | Ha, VL., et al. 2008. J Biol Chem. 283: 14915-26. PMID: 18400762
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDEFL1 Antibody (C-3) | sc-514132 | 200 µg/ml | $322.00 | |||
DDEFL1 Antibody (C-3): m-IgG Fc BP-HRP Bundle | sc-527856 | 200 µg Ab; 10 µg BP | $361.00 | |||
DDEFL1 Antibody (C-3): m-IgG1 BP-HRP Bundle | sc-533229 | 200 µg Ab; 20 µg BP | $361.00 | |||
DDEFL1 Antibody (C-3) AC | sc-514132 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DDEFL1 Antibody (C-3) HRP | sc-514132 HRP | 200 µg/ml | $322.00 | |||
DDEFL1 Antibody (C-3) FITC | sc-514132 FITC | 200 µg/ml | $336.00 | |||
DDEFL1 Antibody (C-3) PE | sc-514132 PE | 200 µg/ml | $349.00 | |||
DDEFL1 Antibody (C-3) Alexa Fluor® 488 | sc-514132 AF488 | 200 µg/ml | $364.00 | |||
DDEFL1 Antibody (C-3) Alexa Fluor® 546 | sc-514132 AF546 | 200 µg/ml | $364.00 | |||
DDEFL1 Antibody (C-3) Alexa Fluor® 594 | sc-514132 AF594 | 200 µg/ml | $364.00 | |||
DDEFL1 Antibody (C-3) Alexa Fluor® 647 | sc-514132 AF647 | 200 µg/ml | $364.00 | |||
DDEFL1 Antibody (C-3) Alexa Fluor® 680 | sc-514132 AF680 | 200 µg/ml | $364.00 | |||
DDEFL1 Antibody (C-3) Alexa Fluor® 790 | sc-514132 AF790 | 200 µg/ml | $364.00 | |||
DDEFL1 (C-3) Neutralizing Peptide | sc-514132 P | 100 µg/0.5 ml | $69.00 |