DDEF2 Antibody (C-9): sc-374323

DDEF2 Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects DDEF2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-DDEF2 antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDEF2, also known as ADP ribosylation factor GTPase-activating protein, plays a crucial role in regulating membrane traffic and cytoskeletal organization by modulating the activity of ARF family GTP-binding proteins. This regulation is vital for the assembly and organization of focal adhesions, which are essential for cell adhesion and signaling. DDEF2 interacts with protein-tyrosine kinases such as Src and focal adhesion kinase, influencing the recruitment and organization of focal adhesion proteins like paxillin and FAK. DDEF2 is primarily localized at peripheral focal adhesions in spreading platelets, where Pyk2 phosphorylates DDEF2 on specific tyrosine residues, affecting its phosphoinositide binding profile. DDEF2 phosphorylation depends on a proline-rich class II Src SH3 binding site, highlighting the importance of post-translational modifications in its function and interactions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DDEF2 Antibody (C-9) References:

1. The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton.  |  Randazzo, PA., et al. 2000. Proc Natl Acad Sci U S A. 97: 4011-6. PMID: 10725410
2. Phosphoinositide-dependent activation of the ADP-ribosylation factor GTPase-activating protein ASAP1. Evidence for the pleckstrin homology domain functioning as an allosteric site.  |  Kam, JL., et al. 2000. J Biol Chem. 275: 9653-63. PMID: 10734117
3. DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism.  |  Furman, C., et al. 2002. J Biol Chem. 277: 7962-9. PMID: 11773070
4. The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.  |  Liu, Y., et al. 2002. Mol Biol Cell. 13: 2147-56. PMID: 12058076
5. CrkL directs ASAP1 to peripheral focal adhesions.  |  Oda, A., et al. 2003. J Biol Chem. 278: 6456-60. PMID: 12522101
6. The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1.  |  Kruljac-Letunic, A., et al. 2003. J Biol Chem. 278: 29560-70. PMID: 12771146
7. Differences between AGAP1, ASAP1 and Arf GAP1 in substrate recognition: interaction with the N-terminus of Arf1.  |  Yoon, HY., et al. 2004. Cell Signal. 16: 1033-44. PMID: 15212764
8. Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling.  |  Liu, Y., et al. 2005. J Biol Chem. 280: 8884-92. PMID: 15632162
9. Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains.  |  Che, MM., et al. 2005. Cell Signal. 17: 1276-88. PMID: 16038802
10. ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src.  |  Brown, MT., et al. 1998. Mol Cell Biol. 18: 7038-51. PMID: 9819391

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDEF2 Antibody (C-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-DDEF2 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDEF2 Antibody (C-9) Specifications

Alternate Product Name: DDEF2

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: DDEF2

Epitope Species: human

Product Reactivity/Detection: DDEF2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for DDEF2 Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin