DDB1 Antibody (C-2): sc-137132
- DDB1 Antibody (C-2) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-300 of DDB1 of human origin
- recommended for detection of DDB1 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDB1 Antibody (C-2) for WB applications. These reagents are now offered in bundles with DDB1 Antibody (C-2) (see ordering information below).
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DDB1 Antibody (C-2) is a mouse monoclonal IgG1 antibody that detects DDB1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDB1, a crucial component of the damaged DNA binding protein complex, plays a vital role in the cellular response to DNA damage, particularly in the repair of ultraviolet light-induced lesions. DDB1 is primarily located in the nucleus, where DDB1 participates in the nucleotide excision repair pathway, ensuring the integrity of the genome by recognizing and binding to damaged DNA sites. Nuclear localization of DDB1 enables timely repair of DNA, preventing mutations that could lead to cancer and other genetic disorders. DDB1 interacts with various proteins, including DDB2, to form a heterodimer essential for the recognition of cyclobutane pyrimidine dimers, a common form of UV-induced DNA damage. Mutations in the DDB2 gene can lead to xeroderma pigmentosum group E, underscoring the critical nature of DDB1′s function in maintaining genomic stability. DDB1 facilitates the repair of UV-induced DNA damage, making DDB1 essential for cellular health and a potential biomarker for understanding the mechanisms underlying skin cancer and other UV-related pathologies.
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DDB1 Antibody (C-2) References:
- Damage-specific DNA binding protein 1 (DDB1): a protein with a wide range of functions. | Iovine, B., et al. 2011. Int J Biochem Cell Biol. 43: 1664-7. PMID: 21959250
- Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide. | Fischer, ES., et al. 2014. Nature. 512: 49-53. PMID: 25043012
- Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation. | Lv, L., et al. 2020. Elife. 9: PMID: 32804079
- DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity. | Wang, X., et al. 2021. Cell Rep. 35: 109281. PMID: 34161765
- Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine. | Zhang, Y., et al. 2021. J Clin Invest. 131: PMID: 34720086
- CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. | Luo, D., et al. 2023. Exp Hematol Oncol. 12: 77. PMID: 37679762
- Structure of the DDB1-AMBRA1 E3 ligase receptor complex linked to cell cycle regulation. | Liu, M., et al. 2023. Nat Commun. 14: 7631. PMID: 37993427
- Targeted Protein Degradation through Recruitment of the CUL4 Complex Adaptor Protein DDB1. | Meyers, M., et al. 2024. ACS Chem Biol. 19: 58-68. PMID: 38192078
- Dual-site molecular glues for enhancing protein-protein interactions of the CDK12-DDB1 complex. | Zhang, Z., et al. 2024. Nat Commun. 15: 6477. PMID: 39090085
- Molecular glue triggers degradation of PHGDH by enhancing the interaction between DDB1 and PHGDH. | Huang, Z., et al. 2024. Acta Pharm Sin B. 14: 4001-4013. PMID: 39309493
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDB1 Antibody (C-2) | sc-137132 | 200 µg/ml | $322.00 | |||
DDB1 Antibody (C-2): m-IgG Fc BP-HRP Bundle | sc-537406 | 200 µg Ab; 10 µg BP | $361.00 | |||
DDB1 Antibody (C-2): m-IgGκ BP-HRP Bundle | sc-534661 | 200 µg Ab; 40 µg BP | $361.00 | |||
DDB1 Antibody (C-2): m-IgG1 BP-HRP Bundle | sc-545175 | 200 µg Ab; 20 µg BP | $361.00 |