DD1-4 Antibody (H-8): sc-390419

DD1-4 Antibody (H-8) is a mouse monoclonal IgG2a antibody that detects dihydrodiol dehydrogenase (DD1, DD2, DD3, and DD4) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-dihydrodiol dehydrogenase antibody (H-8) recognizes multiple isoforms of dihydrodiol dehydrogenase, which are part of the aldo-oxo reductase/aldo-keto reductase superfamily and play a crucial role in the metabolism of xenobiotics, including polycyclic aromatic hydrocarbons, pesticides, and steroid hormones. These enzymes utilize NADH or NADPH as cofactors to catalyze the reduction of ketone-containing drugs, with DD1 and DD2 specifically functioning as 20α-hydroxysteroid dehydrogenases that convert progesterone into its inactive form, 20α-hydroxyprogesterone. Notably, DD3 and DD4 serve as 3α-hydroxysteroid dehydrogenases, with DD3 uniquely capable of catalyzing the dehydrogenation of trans-benzenedihydrodiol and trans-naphthalenedihydrodiol. The expression patterns of these isoforms vary, as DD1 and DD2 are ubiquitously expressed, while DD4 mRNA is primarily found in the liver, highlighting the tissue-specific roles of these enzymes in steroid metabolism and detoxification processes. Anti-dihydrodiol dehydrogenase antibody (H-8) targeting of these isoforms makes DD1-4 an invaluable tool for researchers studying the biochemical pathways involving dihydrodiol dehydrogenases and their implications in human health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DD1-4 Antibody (H-8) References:

1. Development of biocatalysts carrying naphthalene dioxygenase and dihydrodiol dehydrogenase genes inducible in aerobic and anaerobic conditions.  |  Gennaro, PD., et al. 2000. Res Microbiol. 151: 383-91. PMID: 10919518
2. Increased expression of dihydrodiol dehydrogenase induces resistance to cisplatin in human ovarian carcinoma cells.  |  Deng, HB., et al. 2002. J Biol Chem. 277: 15035-43. PMID: 11842089
3. Reduction of dihydrodiol dehydrogenase expression in resected hepatocellular carcinoma.  |  Yang, MD., et al. 2003. Oncol Rep. 10: 271-6. PMID: 12579257
4. Inverse expression of dihydrodiol dehydrogenase and glutathione-S-transferase in patients with esophageal squamous cell carcinoma.  |  Wang, LS., et al. 2004. Int J Cancer. 111: 246-51. PMID: 15197778
5. Purification and characterization of an arene cis-dihydrodiol dehydrogenase endowed with broad substrate specificity toward polycyclic aromatic hydrocarbon dihydrodiols.  |  Jouanneau, Y. and Meyer, C. 2006. Appl Environ Microbiol. 72: 4726-34. PMID: 16820465
6. Study on dihydrodiol dehydrogenase (II) modulation of dihydrodiol dehydrogenase activity by biological disulfides.  |  Terada, T., et al. 1991. Adv Exp Med Biol. 284: 177-85. PMID: 2053475
7. Investigating terephthalate biodegradation: structural characterization of a putative decarboxylating cis-dihydrodiol dehydrogenase.  |  Bains, J., et al. 2012. J Mol Biol. 423: 284-93. PMID: 22889862
8. Guinea-pig liver testosterone 17 beta-dehydrogenase (NADP+) and aldehyde reductase exhibit benzene dihydrodiol dehydrogenase activity.  |  Hara, A., et al. 1985. Biochem J. 225: 177-81. PMID: 2983661
9. Structure of 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase complexed with NADP+.  |  Bennett, MJ., et al. 1996. Biochemistry. 35: 10702-11. PMID: 8718859
10. Sequence of the cDNA of a human dihydrodiol dehydrogenase isoform (AKR1C2) and tissue distribution of its mRNA.  |  Shiraishi, H., et al. 1998. Biochem J. 334 (Pt 2): 399-405. PMID: 9716498

The preferred DD antibody is DD Antibody (C-12): sc-166297.