DD Antibody (C-12): sc-166297
- DD Antibody (C-12) is a mouse monoclonal IgG2b κ DD antibody, cited in 9 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 11-45 near the N-terminus of DD3 of human origin
- DD Antibody (C-12) is recommended for detection of DD1-4 of human origin and AKR1C6 and AKR1C18 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DD Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DD Antibody (C-12) for WB and IHC(P) applications. These reagents are now offered in bundles with DD Antibody (C-12) (see ordering information below).
DD Antibody (C-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects dihydrodiol dehydrogenase (DD) in human, mouse, and rat samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-DD antibody (C-12) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Dihydrodiol dehydrogenases, which include isoforms DD1, DD2, DD3, and DD4, are part of the aldo-oxo reductase/aldo-keto reductase superfamily and play a crucial role in the metabolism of xenobiotics, including polycyclic aromatic hydrocarbons, pesticides, and steroid hormones. These enzymes utilize NADH or NADPH as cofactors to reduce ketone-containing drugs, with DD1 and DD2 specifically catalyzing the conversion of progesterone to its inactive form, 20α-hydroxyprogesterone. DD3 is unique in its ability to catalyze the dehydrogenation of trans-benzenedihydrodiol and trans-naphthalenedihydrodiol, highlighting the diverse functional capabilities of these enzymes. Expression patterns of these isoforms vary, with DD1 and DD2 being ubiquitously expressed, while DD4 mRNA is primarily found in the liver, indicating a specialized role in hepatic metabolism. DD enzymes′ ability to modulate steroid hormone activity and detoxify harmful compounds underscores their importance in both physiological and pathological processes, making DD (C-12) antibody an essential tool for researchers studying these critical metabolic pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DD Antibody (C-12) References:
- Characterization of a novel variant (S145C/L311V) of 3alpha-hydroxysteroid/dihydrodiol dehydrogenase in human liver. | Kume, T., et al. 1999. Pharmacogenetics. 9: 763-71. PMID: 10634139
- Close kinship of human 20alpha-hydroxysteroid dehydrogenase gene with three aldo-keto reductase genes. | Nishizawa, M., et al. 2000. Genes Cells. 5: 111-25. PMID: 10672042
- A novel dihydrodiol dehydrogenase in bovine liver cytosol: purification and characterization of multiple forms of dihydrodiol dehydrogenase. | Mizoguchi, T., et al. 1992. J Biochem. 112: 523-9. PMID: 1491006
- Human hepatic 3 alpha-hydroxysteroid dehydrogenase: possible identity with human hepatic chlordecone reductase. | Binstock, JM., et al. 1992. Biochem Biophys Res Commun. 187: 760-6. PMID: 1530633
- Dihydrodiol dehydrogenase in drug resistance and sensitivity of human carcinomas. | Pan, BF. and Nelson, JA. 2007. Cancer Chemother Pharmacol. 59: 697-702. PMID: 17009030
- Fjord-region benzo[g]chrysene-11,12-dihydrodiol and benzo[c]phenanthrene-3,4-dihydrodiol as substrates for rat liver dihydrodiol dehydrogenase (AKR1C9): structural basis for stereochemical preference. | Shultz, CA., et al. 2008. Chem Res Toxicol. 21: 668-77. PMID: 18251511
- Spectroscopic identification of ortho-quinones as the products of polycyclic aromatic trans-dihydrodiol oxidation catalyzed by dihydrodiol dehydrogenase. A potential route of proximate carcinogen metabolism. | Smithgall, TE., et al. 1988. J Biol Chem. 263: 1814-20. PMID: 3276678
- Enzymatic characterization of a novel bovine liver dihydrodiol dehydrogenase--reaction mechanism and bile acid dehydrogenase activity. | Nanjo, H., et al. 1995. Biochim Biophys Acta. 1244: 53-61. PMID: 7766669
- Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization. | Khanna, M., et al. 1995. Genomics. 25: 588-90. PMID: 7789999
- Dihydrodiol dehydrogenase and its role in polycyclic aromatic hydrocarbon metabolism. | Penning, TM. 1993. Chem Biol Interact. 89: 1-34. PMID: 8221964
- Relationship of human liver dihydrodiol dehydrogenases to hepatic bile-acid-binding protein and an oxidoreductase of human colon cells. | Hara, A., et al. 1996. Biochem J. 313 (Pt 2): 373-6. PMID: 8573067
- Sequence of the cDNA of a human dihydrodiol dehydrogenase isoform (AKR1C2) and tissue distribution of its mRNA. | Shiraishi, H., et al. 1998. Biochem J. 334 (Pt 2): 399-405. PMID: 9716498
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DD Antibody (C-12) | sc-166297 | 200 µg/ml | $322.00 | |||
DD Antibody (C-12): m-IgGκ BP-HRP Bundle | sc-521560 | 200 µg Ab, 40 µg BP | $361.00 | |||
DD Antibody (C-12): m-IgG2b BP-HRP Bundle | sc-548928 | 200 µg Ab; 10 µg BP | $361.00 | |||
DD Antibody (C-12) AC | sc-166297 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DD Antibody (C-12) HRP | sc-166297 HRP | 200 µg/ml | $322.00 | |||
DD Antibody (C-12) FITC | sc-166297 FITC | 200 µg/ml | $336.00 | |||
DD Antibody (C-12) PE | sc-166297 PE | 200 µg/ml | $349.00 | |||
DD Antibody (C-12) Alexa Fluor® 488 | sc-166297 AF488 | 200 µg/ml | $364.00 | |||
DD Antibody (C-12) Alexa Fluor® 546 | sc-166297 AF546 | 200 µg/ml | $364.00 | |||
DD Antibody (C-12) Alexa Fluor® 594 | sc-166297 AF594 | 200 µg/ml | $364.00 | |||
DD Antibody (C-12) Alexa Fluor® 647 | sc-166297 AF647 | 200 µg/ml | $364.00 | |||
DD Antibody (C-12) Alexa Fluor® 680 | sc-166297 AF680 | 200 µg/ml | $364.00 | |||
DD Antibody (C-12) Alexa Fluor® 790 | sc-166297 AF790 | 200 µg/ml | $364.00 | |||
DD (C-12) Neutralizing Peptide | sc-166297 P | 100 µg/0.5 ml | $69.00 |