Dcp1a Antibody (56-Y): sc-100706

Dcp1a Antibody (56-Y) is a mouse monoclonal IgG2a kappa light chain antibody that detects Dcp1a protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Dcp1a antibody (56-Y) is available as a non-conjugated monoclonal isotype antibody. Dcp1a plays a crucial role in regulating mRNA decay, particularly in the major 5′-to-3′ decay pathway and nonsense-mediated decay, which are essential for maintaining cellular RNA integrity and preventing faulty transcript accumulation. Dcp1a is part of a decapping complex including Dcp1 and Dcp2, responsible for removing the 5′-cap structure from mRNA, a critical step in mRNA degradation. In humans, Dcp1a and hDcp2 are recruited to mRNAs with premature termination codons by Upf proteins, facilitating defective mRNA degradation. Dcp1a colocalizes with hDcp2 in the cytoplasm, and their interaction enables effective mRNA turnover. Additionally, Dcp1a interacts with Smad4, forming a complex essential for nuclear translocation of Dcp1a in response to TGFβ signaling. This interaction occurs through a specific domain on Dcp1a and a proline-rich activation domain on Smad4, highlighting Dcp1a′s importance in cellular signaling pathways and various biological processes.

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Dcp1a Antibody (56-Y) References:

1. Mechanisms and control of mRNA decapping in Saccharomyces cerevisiae.  |  Tucker, M. and Parker, R. 2000. Annu Rev Biochem. 69: 571-95. PMID: 10966469
2. Signaling of transforming growth factor-beta family members through Smad proteins.  |  Itoh, S., et al. 2000. Eur J Biochem. 267: 6954-67. PMID: 11106403
3. Smad regulation in TGF-beta signal transduction.  |  Moustakas, A., et al. 2001. J Cell Sci. 114: 4359-69. PMID: 11792802
4. SMIF, a Smad4-interacting protein that functions as a co-activator in TGFbeta signalling.  |  Bai, RY., et al. 2002. Nat Cell Biol. 4: 181-90. PMID: 11836524
5. Review of current progress in the structure and function of Smad proteins.  |  Chen, W., et al. 2002. Chin Med J (Engl). 115: 446-50. PMID: 11940386
6. An EVH1/WH1 domain as a key actor in TGFbeta signalling.  |  Callebaut, I. 2002. FEBS Lett. 519: 178-80. PMID: 12023040
7. Initiation-mediated mRNA decay in yeast affects heat-shock mRNAs, and works through decapping and 5'-to-3' hydrolysis.  |  Heikkinen, HL., et al. 2003. Nucleic Acids Res. 31: 4006-16. PMID: 12853617
8. Endonuclease-mediated mRNA decay involves the selective targeting of PMR1 to polyribosome-bound substrate mRNA.  |  Yang, F. and Schoenberg, DR. 2004. Mol Cell. 14: 435-45. PMID: 15149593
9. Decapping reaction of mRNA requires Dcp1 in fission yeast: its characterization in different species from yeast to human.  |  Sakuno, T., et al. 2004. J Biochem. 136: 805-12. PMID: 15671491
10. Expression of DCP1a in gastric cancer and its biological function and mechanism in chemotherapy resistance in gastric cancer cells.  |  Ruan, T., et al. 2020. Dig Liver Dis. 52: 1351-1358. PMID: 32646734
11. DCP1A is an unfavorable prognostic-related enhancer RNA in hepatocellular carcinoma.  |  Wu, H., et al. 2021. Aging (Albany NY). 13: 23020-23035. PMID: 34609335
12. Isolation and characterization of Dcp1p, the yeast mRNA decapping enzyme.  |  LaGrandeur, TE. and Parker, R. 1998. EMBO J. 17: 1487-96. PMID: 9482745

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dcp1a Antibody (56-Y) has 41 citations in a variety of scientific publications.

Primary antibodies like Anti-Dcp1a Antibody (56-Y) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dcp1a Antibody (56-Y) Specifications

Alternate Product Name: Dcp1a

Product Clone Name: 56-Y

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 186-286

Epitope Target: Dcp1a

Epitope Species: human

Product Reactivity/Detection: Dcp1a

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 50 µg IgG_2a kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 41 citations in various scientific publications

Available Conjugates/Forms for Dcp1a Antibody (56-Y): N/A