DAZL Antibody (E-6): sc-390929
- DAZL Antibody (E-6) is a mouse monoclonal IgG2a κ DAZL antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 206-295 mapping at the C-terminus of DAZL of human origin
- DAZL Antibody (E-6) is recommended for detection of DAZL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DAZL Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DAZL Antibody (E-6) for WB and IHC(P) applications. These reagents are now offered in bundles with DAZL Antibody (E-6) (see ordering information below).
QUICK LINKS
DAZL Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects DAZL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-DAZL antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DAZL (E-6) antibody recognizes deleted in azoospermia-like protein, which plays a crucial role in spermatogenesis as an RNA-binding protein, influencing the development and maturation of germ cells. DAZL expression is restricted to germ cells in both male and female gonads, highlighting its importance in fertility. The gene encoding human DAZL is located on chromosome 3p24 and shares a highly conserved RNA-binding motif with other members of the DAZ family, such as BOULE and DAZ. A mutation at Thr 54 to Ala within the RNA-recognition domain of DAZL can lead to spermatogenic failure, emphasizing DAZL′s critical function in reproductive health. Infertility observed in DAZL knockout mice is attributed to their germ cells′ inability to complete the first meiotic prophase during the initial wave of spermatogenesis, underscoring DAZL′s essential role in male fertility.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DAZL Antibody (E-6) References:
- Localization of a specific germ cell marker, DAZL1, in testicular germ cell neoplasias. | Lifschitz-Mercer, B., et al. 2002. Virchows Arch. 440: 387-91. PMID: 11956819
- Association of a single-nucleotide polymorphism of the deleted-in-azoospermia-like gene with susceptibility to spermatogenic failure. | Teng, YN., et al. 2002. J Clin Endocrinol Metab. 87: 5258-64. PMID: 12414900
- Expression profiling of the developing testis in wild-type and Dazl knockout mice. | Maratou, K., et al. 2004. Mol Reprod Dev. 67: 26-54. PMID: 14648873
- Expression profiles of the DAZ gene family in human testis with and without spermatogenic failure. | Kuo, PL., et al. 2004. Fertil Steril. 81: 1034-40. PMID: 15066460
- DAZL polymorphisms and susceptibility to spermatogenic failure: an example of remarkable ethnic differences. | Becherini, L., et al. 2004. Int J Androl. 27: 375-81. PMID: 15595957
- Identification and characterization of RNA sequences to which human PUMILIO-2 (PUM2) and deleted in Azoospermia-like (DAZL) bind. | Fox, M., et al. 2005. Genomics. 85: 92-105. PMID: 15607425
- Role of the DAZ genes in male fertility. | Reynolds, N. and Cooke, HJ. 2005. Reprod Biomed Online. 10: 72-80. PMID: 15705297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAZL Antibody (E-6) | sc-390929 | 200 µg/ml | $322.00 | |||
DAZL Antibody (E-6): m-IgG Fc BP-HRP Bundle | sc-530306 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAZL Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-523739 | 200 µg Ab, 40 µg BP | $361.00 | |||
DAZL Antibody (E-6): m-IgG2a BP-HRP Bundle | sc-547478 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAZL Antibody (E-6) AC | sc-390929 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DAZL Antibody (E-6) HRP | sc-390929 HRP | 200 µg/ml | $322.00 | |||
DAZL Antibody (E-6) FITC | sc-390929 FITC | 200 µg/ml | $336.00 | |||
DAZL Antibody (E-6) PE | sc-390929 PE | 200 µg/ml | $349.00 | |||
DAZL Antibody (E-6) Alexa Fluor® 488 | sc-390929 AF488 | 200 µg/ml | $364.00 | |||
DAZL Antibody (E-6) Alexa Fluor® 546 | sc-390929 AF546 | 200 µg/ml | $364.00 | |||
DAZL Antibody (E-6) Alexa Fluor® 594 | sc-390929 AF594 | 200 µg/ml | $364.00 | |||
DAZL Antibody (E-6) Alexa Fluor® 647 | sc-390929 AF647 | 200 µg/ml | $364.00 | |||
DAZL Antibody (E-6) Alexa Fluor® 680 | sc-390929 AF680 | 200 µg/ml | $364.00 | |||
DAZL Antibody (E-6) Alexa Fluor® 790 | sc-390929 AF790 | 200 µg/ml | $364.00 |