Daxx Antibody (H-7): sc-8043

Daxx Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Daxx protein of mouse, rat, and human origin through western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. Daxx (H-7) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Daxx plays a crucial role in the regulation of apoptosis, particularly in the context of the FAS-mediated apoptotic pathway. When the FAS receptor is activated by its ligand, activation triggers a cascade of events leading to programmed cell death, which is essential for maintaining immune system balance and tissue homeostasis. Daxx is a key player in this process, as Daxx interacts with the death domain of the FAS receptor, facilitating the activation of Jun N-terminal kinase (JNK) and promoting apoptosis. Notably, while Daxx does not possess a death domain, Daxx overexpression can enhance FAS-mediated apoptosis by activating the JNK pathway. This pathway operates alongside, yet distinctly from, the FAS-FADD-FLICE pathway, which is another critical route for apoptosis. The Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2, highlighting unique regulatory mechanisms in cell death.

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Daxx Antibody (H-7) References:

1. Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx.  |  Park, J., et al. 2007. J Mol Biol. 368: 388-97. PMID: 17362989
2. Anti-apoptotic effects of probucol are associated with downregulation of Daxx expression in THP-1 macrophage.  |  Su, B., et al. 2007. Cardiovasc Drugs Ther. 21: 37-45. PMID: 17370120
3. DAXX-ATRX regulation of p53 chromatin binding and DNA damage response.  |  Gulve, N., et al. 2022. Nat Commun. 13: 5033. PMID: 36028493
4. DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network.  |  Carraro, M., et al. 2023. Mol Cell. 83: 1075-1092.e9. PMID: 36868228
5. DAXX promotes centromeric stability independently of ATRX by preventing the accumulation of R-loop-induced DNA double-stranded breaks.  |  Pinto, LM., et al. 2024. Nucleic Acids Res. 52: 1136-1155. PMID: 38038252
6. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis.  |  Chinnaiyan, AM., et al. 1995. Cell. 81: 505-12. PMID: 7538907
7. TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways.  |  Hsu, H., et al. 1996. Cell. 84: 299-308. PMID: 8565075
8. A license to kill.  |  Fraser, A. and Evan, G. 1996. Cell. 85: 781-4. PMID: 8681372
9. Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.  |  Boldin, MP., et al. 1996. Cell. 85: 803-15. PMID: 8681376
10. Apoptosis by death factor.  |  Nagata, S. 1997. Cell. 88: 355-65. PMID: 9039262
11. Mitogen-activated protein kinase-mediated Fas apoptotic signaling pathway.  |  Goillot, E., et al. 1997. Proc Natl Acad Sci U S A. 94: 3302-7. PMID: 9096388
12. Daxx, a novel Fas-binding protein that activates JNK and apoptosis.  |  Yang, X., et al. 1997. Cell. 89: 1067-76. PMID: 9215629

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Daxx Antibody (H-7) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-Daxx Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Daxx Antibody (H-7) Specifications

Alternate Product Name: Death-associated protein 6 (DAP6)

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 627-739

Epitope Target: Daxx

Epitope Species: mouse

Product Reactivity/Detection: Daxx

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for Daxx Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin