Daxx Antibody (H-7) recommended for detection of Daxx of mouse, rat and human origin by WB, IP, IF and ELISA

Daxx Antibody (H-7): sc-8043

Daxx Antibody (H-7) is rated 4.2 out of 5 by 20.
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Datasheets

    • Anti-Daxx Antibody (H-7) is a mouse monoclonal IgG2a (kappa light chain) Daxx antibody provided at 200 µg/ml
    • raised against amino acids 627-739 mapping at the C-terminus of Daxx of mouse origin
    • Anti-Daxx Antibody (H-7) is recommended for detection of Daxx of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Daxx Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Daxx (H-7): sc-8043.

What is the recommended starting dilution for Western blot for Daxx (H-7): sc-8043?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-8043.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 14
Date published: 2019-02-28
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Rated 5 out of 5 by from It worked well for IF in mouse ES cells It worked well for conventional IF with low background in mouse ES cells.
Date published: 2020-09-29
Rated 4 out of 5 by from Work for mouse breast cancer cell lines Work for IF and WB in mouse. The signal is clear but relatively weak.
Date published: 2018-02-20
Rated 1 out of 5 by from Daxx ab for wb I got free sample from this ab and tested with 5 different mouse cell lines and unfortunately it did not work for me.
Date published: 2017-12-07
Rated 3 out of 5 by from Daxx Signal was good, detected molecular weight less than 130 KDA in Esophageal cancer cell
Date published: 2017-11-13
Rated 5 out of 5 by from very nice quality It has been reported in the literature that the expression of Daxx protein in gastric cancer is very high. We used this antibody to detect the expression of Daxx. The specificity of this expression is very good. After 1:500 dilution, the target band 120kda obtained
Date published: 2017-09-16
Rated 5 out of 5 by from This antibody can get clear bands. In our lab, this antibody has good quality in Western blotting. Using 1:500 I get clear bands.
Date published: 2017-06-06
Rated 5 out of 5 by from strong signal Daxx antibody used in IF . Observed strong excellent signal.
Date published: 2017-06-03
Rated 5 out of 5 by from Antibodies used Rat , Western Blotting Antibodies in the laboratory for two years, Western Blotting diluted to 1:1000, still get the desired results.
Date published: 2017-06-01
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