DARPP-32 Antibody (H-3): sc-271111

DARPP-32 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects DARPP-32 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DARPP-32 (H-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DARPP-32 protein, also known as dopamine and adenosine 3′,5′-monophosphate-regulated phosphoprotein, plays a crucial role in dopaminergic signaling pathways, which are vital for numerous brain functions, including motor control, reward processing, and cognitive functions. Located predominantly in neurons that receive dopaminergic input, DARPP-32 acts as a key integrator of dopamine signaling, where phosphorylation state is critical for modulating the activity of protein phosphatase 1, a major enzyme involved in synaptic plasticity and memory formation. Phosphorylation of DARPP-32 on threonine is stimulated by dopamine binding to D1-like receptors, while D2-like receptor activation leads to dephosphorylation, highlighting the intricate balance of signaling pathways that govern neuronal function. Abnormalities in DARPP-32 signaling have been implicated in various neurological disorders, including schizophrenia, Parkinson′s disease, and substance abuse, making DARPP-32 an important target for research and therapeutic interventions.

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DARPP-32 Antibody (H-3) References:

1. The significance of L-amino acid decarboxylase and DARPP-32 in the kidney.  |  Aperia, A., et al. 1990. Am J Hypertens. 3: 11S-13S. PMID: 2166532
2. DARPP-32 as a marker for D-1 dopaminoceptive cells in the rat brain: prenatal development and presence in glial elements (tanycytes) in the basal hypothalamus.  |  Hökfelt, T., et al. 1988. Adv Exp Med Biol. 235: 65-82. PMID: 2976255
3. DARPP-32 and t-DARPP promote non-small cell lung cancer growth through regulation of IKKα-dependent cell migration.  |  Alam, SK., et al. 2018. Commun Biol. 1: 43. PMID: 29782621
4. Activation of IGF1R by DARPP-32 promotes STAT3 signaling in gastric cancer cells.  |  Zhu, S., et al. 2019. Oncogene. 38: 5805-5816. PMID: 31235784
5. Unravelling transcriptomic complexity in breast cancer through modulation of DARPP-32 expression and signalling pathways.  |  Saidy, B., et al. 2023. Sci Rep. 13: 21163. PMID: 38036593
6. Darpp-32 is regulated by dopamine and is required for the formation of GABAergic neurons in the developing telencephalon.  |  Souza, BR., et al. 2024. Prog Neuropsychopharmacol Biol Psychiatry. 134: 111060. PMID: 38906412
7. DARPP-32, a dopamine-regulated neuronal phosphoprotein, is a potent inhibitor of protein phosphatase-1.  |  Hemmings, HC., et al. Nature. 310: 503-5. PMID: 6087160
8. DARPP-32, a dopamine- and adenosine 3':5'-monophosphate-regulated phosphoprotein enriched in dopamine-innervated brain regions. I. Regional and cellular distribution in the rat brain.  |  Walaas, SI. and Greengard, P. 1984. J Neurosci. 4: 84-98. PMID: 6319627
9. DARPP-32, a dopamine- and adenosine 3':5'-monophosphate-regulated neuronal phosphoprotein. I. Amino acid sequence around the phosphorylated threonine.  |  Hemmings, HC., et al. 1984. J Biol Chem. 259: 14486-90. PMID: 6501302
10. Bidirectional regulation of DARPP-32 phosphorylation by dopamine.  |  Nishi, A., et al. 1997. J Neurosci. 17: 8147-55. PMID: 9334390
11. The DARPP-32/protein phosphatase-1 cascade: a model for signal integration.  |  Greengard, P., et al. 1998. Brain Res Brain Res Rev. 26: 274-84. PMID: 9651542
12. DARPP-32: regulator of the efficacy of dopaminergic neurotransmission.  |  Fienberg, AA., et al. 1998. Science. 281: 838-42. PMID: 9694658

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DARPP-32 Antibody (H-3) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-DARPP-32 Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DARPP-32 Antibody (H-3) Specifications

Alternate Product Name: DARPP-32

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-34

Epitope Target: DARPP-32

Epitope Species: human

Product Reactivity/Detection: DARPP-32

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for DARPP-32 Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin