DARPP-32 Antibody (G-5): sc-398360
- DARPP-32 Antibody (G-5) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 134-195 mapping epitope corresponding to amino acids 134-195 of DARPP-32 of bovine origin
- recommended for detection of DARPP-32 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
- See DARPP-32 (H-3): sc-271111 for DARPP-32 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DARPP-32 Antibody (G-5) for WB and IHC(P) applications. These reagents are now offered in bundles with DARPP-32 Antibody (G-5) (see ordering information below).
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DARPP-32 Antibody (G-5) is a mouse monoclonal IgG2a antibody that detects DARPP-32 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DARPP-32, or dopamine and adenosine 3′,5′-monophosphate-regulated phosphoprotein, plays a crucial role in dopaminergic signaling pathways, which are vital for numerous brain functions and are often disrupted in neurological disorders such as schizophrenia, Parkinson′s disease, and substance abuse. DARPP-32 is predominantly located in the cytoplasm of neurons that receive dopaminergic input, where DARPP-32 acts as a key regulator of dopamine signaling. The spatial distribution of DARPP-32 is significant because DARPP-32 allows for the integration of signals from both D1-like and D2-like dopamine receptors, facilitating a balanced response to dopamine. Upon activation of D1-like receptors, DARPP-32 is phosphorylated by protein kinase A, which enhances DARPP-32′s ability to inhibit protein phosphatase 1, thereby modulating downstream signaling pathways. Conversely, when D2-like receptors are activated, the inhibition of protein kinase A leads to the dephosphorylation of DARPP-32, demonstrating DARPP-32′s role as a molecular switch in dopaminergic signaling. DARPP-32′s ability to respond to various neurotransmitters further underscores DARPP-32′s importance in maintaining neuronal function and potential as a therapeutic target in treating dopamine-related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DARPP-32 Antibody (G-5) References:
- DARPP-32 mediates serotonergic neurotransmission in the forebrain. | Svenningsson, P., et al. 2002. Proc Natl Acad Sci U S A. 99: 3188-93. PMID: 11880652
- Control of electrolyte transport in the kidney through a dopamine- and cAMP-regulated phosphoprotein, DARPP-32. | Fryckstedt, J., et al. 1992. J Auton Pharmacol. 12: 183-9. PMID: 1321155
- DARPP-32 expression arises after a phase of dysplasia in oesophageal squamous cell carcinoma. | Ebihara, Y., et al. 2004. Br J Cancer. 91: 119-23. PMID: 15188007
- DARPP-32 phosphorylation opposes the behavioral effects of nicotine. | Zhu, H., et al. 2005. Biol Psychiatry. 58: 981-9. PMID: 16084497
- DARPP-32 is a robust integrator of dopamine and glutamate signals. | Fernandez, E., et al. 2006. PLoS Comput Biol. 2: e176. PMID: 17194217
- DARPP-32 increases interactions between epidermal growth factor receptor and ERBB3 to promote tumor resistance to gefitinib. | Zhu, S., et al. 2011. Gastroenterology. 141: 1738-48.e1-2. PMID: 21741919
- Detailed DARPP-32 expression profiles in postmortem brains from patients with schizophrenia: an immunohistochemical study. | Kunii, Y., et al. 2011. Med Mol Morphol. 44: 190-9. PMID: 22179181
- Effects of DARPP-32 Genetic Variation on Prefrontal Cortex Volume and Episodic Memory Performance. | Persson, N., et al. 2017. Front Neurosci. 11: 244. PMID: 28553197
- Silencing of miR490-3p by H. pylori activates DARPP-32 and induces resistance to gefitinib. | Zhu, S., et al. 2020. Cancer Lett. 491: 87-96. PMID: 32735911
- PP1, PKA and DARPP-32 in breast cancer: A retrospective assessment of protein and mRNA expression. | Saidy, B., et al. 2021. J Cell Mol Med. 25: 5015-5024. PMID: 33991172
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DARPP-32 Antibody (G-5) | sc-398360 | 200 µg/ml | $322.00 | |||
DARPP-32 Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-538425 | 200 µg Ab; 10 µg BP | $361.00 | |||
DARPP-32 Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-536038 | 200 µg Ab; 40 µg BP | $361.00 |