Dapper1 Antibody (D-4): sc-377030

Dapper1 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dapper1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Dapper1 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dapper1, also known as DACT1 (dapper, antagonist of beta-catenin, homolog 1), DPR1, HNG3, or FRODO, is an 836 amino acid protein that plays a crucial role in cellular signaling by localizing to both the nucleus and cytoplasm. A C-terminal PDZ-binding motif is essential for mediating protein-protein interactions, particularly with Dishevelled-2 (Dvl-2). Through Dvl-2 interaction, Dapper1 positively regulates developmental signaling pathways by inhibiting β-catenin degradation, which enhances transcriptional activation of target genes within the Wnt signaling pathway. This regulation is vital for proper embryonic development and cellular differentiation. Notably, Dapper1 is downregulated in hepatocellular carcinoma, indicating a potential role in tumor suppression. The gene encoding Dapper1 is located on human chromosome 14, a region containing over 700 genes and implicated in various genetic disorders, including those associated with Alzheimer′s disease and α1-antitrypsin deficiency.

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Dapper1 Antibody (D-4) References:

1. Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation.  |  Cheyette, BN., et al. 2002. Dev Cell. 2: 449-61. PMID: 11970895
2. Identification and characterization of human DAPPER1 and DAPPER2 genes in silico.  |  Katoh, M. and Katoh, M. 2003. Int J Oncol. 22: 907-13. PMID: 12632086
3. HDPR1, a novel inhibitor of the WNT/beta-catenin signaling, is frequently downregulated in hepatocellular carcinoma: involvement of methylation-mediated gene silencing.  |  Yau, TO., et al. 2005. Oncogene. 24: 1607-14. PMID: 15580286
4. Dapper 1 antagonizes Wnt signaling by promoting dishevelled degradation.  |  Zhang, L., et al. 2006. J Biol Chem. 281: 8607-12. PMID: 16446366
5. Dapper1 is a nucleocytoplasmic shuttling protein that negatively modulates Wnt signaling in the nucleus.  |  Gao, X., et al. 2008. J Biol Chem. 283: 35679-88. PMID: 18936100
6. Dact1, a nutritionally regulated preadipocyte gene, controls adipogenesis by coordinating the Wnt/beta-catenin signaling network.  |  Lagathu, C., et al. 2009. Diabetes. 58: 609-19. PMID: 19073771
7. Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation.  |  Ma, B., et al. 2014. Cell Res. 24: 912-24. PMID: 24980960
8. The Wnt Signaling Antagonist Dapper1 Accelerates Dishevelled2 Degradation via Promoting Its Ubiquitination and Aggregate-induced Autophagy.  |  Ma, B., et al. 2015. J Biol Chem. 290: 12346-54. PMID: 25825496
9. Dapper1 attenuates hepatic gluconeogenesis and lipogenesis by activating PI3K/Akt signaling.  |  Kuang, JR., et al. 2017. Mol Cell Endocrinol. 447: 106-115. PMID: 28237722
10. Head formation requires Dishevelled degradation that is mediated by March2 in concert with Dapper1.  |  Lee, H., et al. 2018. Development. 145: PMID: 29549110
11. Cyclin G2 regulates canonical Wnt signalling via interaction with Dapper1 to attenuate tubulointerstitial fibrosis in diabetic nephropathy.  |  Zhao, C., et al. 2020. J Cell Mol Med. 24: 2749-2760. PMID: 31978940

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dapper1 Antibody (D-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Dapper1 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dapper1 Antibody (D-4) Specifications

Alternate Product Name: Dapper1

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 281-460

Epitope Target: Dapper1

Epitope Species: human

Product Reactivity/Detection: Dapper1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Dapper1 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin