DAP12 Antibody (A-4): sc-166084

DAP12 Antibody (A-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects DAP12 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP12 (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DAP12, also known as TYROBP, is a crucial phosphoprotein that plays a significant role in the activation of natural killer (NK) cells, which are essential components of the innate immune system. Located in the plasma membrane, DAP12 interacts with several receptor families, including killer-cell inhibitory receptors (KIR), CD94/NKG2, and Ly-49, facilitating the recognition of major histocompatibility complex (MHC) class I molecules. This interaction is vital for the regulation of NK cell activity, as DAP12 helps to balance stimulatory and inhibitory signals, ultimately influencing immune responses. Notably, DAP12′s phosphorylation allows binding to signaling proteins such as ZAP-70 and Syk, indicating that DAP12′s activation pathway may share similarities with those of T and B cell antigen receptors. DAP12′s ability to form complexes with other proteins, such as CD94/NKG2C, Ly-49D, and Ly-49H, further underscores DAP12′s importance in immune signaling and cellular activation, making anti-DAP12 antibody (A-4) an invaluable tool for researchers studying NK cell biology and immune responses.

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DAP12 Antibody (A-4) References:

1. The ITAM-bearing transmembrane adaptor DAP12 in lymphoid and myeloid cell function.  |  Lanier, LL. and Bakker, AB. 2000. Immunol Today. 21: 611-4. PMID: 11114420
2. The expanding roles of ITAM adapters FcRgamma and DAP12 in myeloid cells.  |  Hamerman, JA., et al. 2009. Immunol Rev. 232: 42-58. PMID: 19909355
3. Structure, expression pattern and biological activity of molecular complex TREM-2/DAP12.  |  Paradowska-Gorycka, A. and Jurkowska, M. 2013. Hum Immunol. 74: 730-7. PMID: 23459077
4. Absence of Dap12 and the αvβ3 integrin causes severe osteopetrosis.  |  Zou, W. and Teitelbaum, SL. 2015. J Cell Biol. 208: 125-36. PMID: 25547154
5. DAP12 deficiency alters microglia-oligodendrocyte communication and enhances resilience against tau toxicity.  |  Chen, H., et al. 2023. bioRxiv.. PMID: 37961594
6. DAP12 deficiency alters microglia-oligodendrocyte communication and enhances resilience against tau toxicity.  |  Chen, H., et al. 2023. Res Sq.. PMID: 37961627
7. TREM1/DAP12 based novel multiple chain CAR-T cells targeting DLL3 show robust anti-tumour efficacy for small cell lung cancer.  |  Nie, F., et al. 2024. Immunology. 172: 362-374. PMID: 38469682
8. Immunoreceptor DAP12 bearing a tyrosine-based activation motif is involved in activating NK cells.  |  Lanier, LL., et al. 1998. Nature. 391: 703-7. PMID: 9490415
9. NK cell receptors.  |  Lanier, LL. 1998. Annu Rev Immunol. 16: 359-93. PMID: 9597134
10. NKp44, a novel triggering surface molecule specifically expressed by activated natural killer cells, is involved in non-major histocompatibility complex-restricted tumor cell lysis.  |  Vitale, M., et al. 1998. J Exp Med. 187: 2065-72. PMID: 9625766
11. Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors.  |  Smith, KM., et al. 1998. J Immunol. 161: 7-10. PMID: 9647200
12. Association of DAP12 with activating CD94/NKG2C NK cell receptors.  |  Lanier, LL., et al. 1998. Immunity. 8: 693-701. PMID: 9655483

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DAP12 Antibody (A-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-DAP12 Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DAP12 Antibody (A-4) Specifications

Alternate Product Name: TYROBP

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 76-112

Epitope Target: DAP12

Epitope Species: human

Product Reactivity/Detection: DAP12

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for DAP12 Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin