DAP-5 Antibody (B-1): sc-374236

DAP-5 Antibody (B-1) is a mouse monoclonal IgG2b antibody that detects Death-associated protein 5 (DAP-5) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP-5 plays a crucial role in translation regulation, particularly during apoptosis, where caspases cleave DAP-5 to produce a C-terminal truncated form that forms complexes with eIF4A and eIF3. This truncated DAP-5 lacks the N-terminal region of eIF4G, which is essential for binding to cap-binding protein eIF4E, thereby allowing DAP-5 to act as a general translation repressor. DAP-5′s ability to mediate cap-independent translation from its own internal ribosome entry site creates a positive feedback loop that sustains expression during cellular stress, highlighting DAP-5′s importance in maintaining cellular homeostasis and promoting specific gene expression pathways necessary for cellular differentiation. Anti-DAP-5 antibody (B-1) serves as an invaluable tool for researchers studying translation regulation and apoptosis mechanisms, providing insights into molecular pathways that govern cell fate decisions.

The preferred DAP-5 antibody is DAP-5 Antibody (F-2): sc-137011.