DAK Antibody (A-5): sc-365458
- DAK Antibody (A-5) is a mouse monoclonal IgG1 κ DAK antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 535-565 near the C-terminus of DAK of human origin
- DAK Antibody (A-5) is recommended for detection of DAK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DAK Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DAK Antibody (A-5). This work is in progress.
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SEE ALSO...
DAK Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects DAK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-DAK antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DAK protein, also known as dihydroxyacetone kinase 2 homolog or NET45, is a bifunctional enzyme that plays a crucial role in cellular metabolism by catalyzing the phosphorylation of dihydroxyacetone and the conversion of FAD to cyclized FMN. This enzymatic activity is vital for energy production and metabolic regulation, as DAK links carbohydrate metabolism to the generation of essential cofactors. DAK is a 575 amino acid protein that exists as a homodimer and contains one DhaK domain and one DhaL domain, with DAK gene located on human chromosome 11, a region associated with various genetic disorders, including Jervell and Lange-Nielsen syndrome and Niemann-Pick disease. DAK′s ability to interact with specific substrates and role in metabolic pathways underscore DAK′s importance in maintaining cellular homeostasis and energy balance, making DAK (A-5) monoclonal antibody an essential tool for researchers studying metabolic diseases and related conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DAK Antibody (A-5) References:
- Smith-Lemli-Opitz syndrome and the DHCR7 gene. | Jira, PE., et al. 2003. Ann Hum Genet. 67: 269-80. PMID: 12914579
- Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases. | Cabezas, A., et al. 2005. Biochem Biophys Res Commun. 338: 1682-9. PMID: 16289032
- The pathogenesis and treatment of acid sphingomyelinase-deficient Niemann-Pick disease. | Schuchman, EH. 2007. J Inherit Metab Dis. 30: 654-63. PMID: 17632693
- Antiproliferative effect of dihydroxyacetone on Trypanosoma brucei bloodstream forms: cell cycle progression, subcellular alterations, and cell death. | Uzcátegui, NL., et al. 2007. Antimicrob Agents Chemother. 51: 3960-8. PMID: 17682096
- Chromosomal microarray mapping suggests a role for BSX and Neurogranin in neurocognitive and behavioral defects in the 11q terminal deletion disorder (Jacobsen syndrome). | Coldren, CD., et al. 2009. Neurogenetics. 10: 89-95. PMID: 18855024
- An intronic mutation leading to incomplete skipping of exon-2 in KCNQ1 rescues hearing in Jervell and Lange-Nielsen syndrome. | Bhuiyan, ZA., et al. 2008. Prog Biophys Mol Biol. 98: 319-27. PMID: 19027783
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAK Antibody (A-5) | sc-365458 | 200 µg/ml | $316.00 | |||
DAK Antibody (A-5) AC | sc-365458 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DAK Antibody (A-5) HRP | sc-365458 HRP | 200 µg/ml | $316.00 | |||
DAK Antibody (A-5) FITC | sc-365458 FITC | 200 µg/ml | $330.00 | |||
DAK Antibody (A-5) PE | sc-365458 PE | 200 µg/ml | $343.00 | |||
DAK Antibody (A-5) Alexa Fluor® 488 | sc-365458 AF488 | 200 µg/ml | $357.00 | |||
DAK Antibody (A-5) Alexa Fluor® 546 | sc-365458 AF546 | 200 µg/ml | $357.00 | |||
DAK Antibody (A-5) Alexa Fluor® 594 | sc-365458 AF594 | 200 µg/ml | $357.00 | |||
DAK Antibody (A-5) Alexa Fluor® 647 | sc-365458 AF647 | 200 µg/ml | $357.00 | |||
DAK Antibody (A-5) Alexa Fluor® 680 | sc-365458 AF680 | 200 µg/ml | $357.00 | |||
DAK Antibody (A-5) Alexa Fluor® 790 | sc-365458 AF790 | 200 µg/ml | $357.00 | |||
DAK (A-5) Neutralizing Peptide | sc-365458 P | 100 µg/0.5 ml | $68.00 |