Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DAGLα CRISPR/Cas9 KO Plasmid (h): sc-402826
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAGLα CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DAGLα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DAGLα Antibody (E-6): sc-390409
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAGLα CRISPR/Cas9 KO Plasmid (h) | sc-402826 | 20 µg | $397.00 |
Overview
DAGLA encodes diacylglycerol lipase alpha (DAGLα), a membrane-associated serine hydrolase that catalyzes the hydrolysis of diacylglycerol to generate 2-arachidonoylglycerol (2-AG), a principal endocannabinoid lipid mediator. By controlling 2-AG production, DAGLα influences retrograde synaptic signaling, CB1 receptor activation, and activity-dependent modulation of neurotransmitter release, linking lipid metabolism to neuronal excitability and plasticity. DAGLα activity interfaces with phospholipase C–diacylglycerol signaling and broader arachidonic acid–related lipid networks that shape inflammation and cellular stress responses. Altered DAGLA-dependent endocannabinoid tone has been associated with neurodevelopmental and neuropsychiatric phenotypes and is studied in contexts such as epilepsy, pain processing, and metabolic dysregulation.
DAGLα CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DAGLA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DAGLA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DAGLA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DAGLα protein expression.
This CRISPR knockout system enables efficient generation of DAGLA-deficient cell models for investigation of DAGLα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DAGLA exon(s) critical for DAGLα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DAGLA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DAGLα CRISPR/Cas9 KO Plasmid (h) and DAGLα CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DAGLA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DAGLα HDR Plasmid (h) and DAGLα HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DAGLA homology arms to support homology-directed repair at defined DAGLA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.