DAGLα Antibody (E-6): sc-390409

DAGLα Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects DAGLα protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DAGLα antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DAGLα, also known as diacylglycerol lipase alpha, NSDDR, or C11orf11, is a 1,042 amino acid multi-pass membrane protein that plays a pivotal role in lipid metabolism as a member of the AB hydrolase superfamily. DAGLα protein is primarily located in the brain and pancreas, where DAGLα utilizes calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) into 2-arachidonoyl-glycerol (2-AG). This reaction is essential for axonal growth and retrograde synaptic signaling at mature synapses, highlighting DAGLα′s importance in neuronal communication and development. The gene encoding DAGLα is situated on human chromosome 11, which contains over 1,400 genes and constitutes nearly 4% of the human genome. DAGLα functions optimally at a pH of 7, and DAGLα′s enzymatic activity can be inhibited by compounds such as p-hydroxy-mercuri-benzoate and mercury chloride, making DAGLα a critical target for studies related to synaptic function and lipid signaling pathways.

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DAGLα Antibody (E-6) References:

1. Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.  |  Nakajima, D., et al. 2002. DNA Res. 9: 99-106. PMID: 12168954
2. Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain.  |  Bisogno, T., et al. 2003. J Cell Biol. 163: 463-8. PMID: 14610053
3. Stimulation of endocannabinoid formation in brain slice cultures through activation of group I metabotropic glutamate receptors.  |  Jung, KM., et al. 2005. Mol Pharmacol. 68: 1196-202. PMID: 16051747
4. Endocannabinoid metabolic pathways and enzymes.  |  Ligresti, A., et al. 2005. Curr Drug Targets CNS Neurol Disord. 4: 615-23. PMID: 16375679
5. Critical enzymes involved in endocannabinoid metabolism.  |  Basavarajappa, BS. 2007. Protein Pept Lett. 14: 237-46. PMID: 17346227
6. Endocannabinoids and synaptic function in the CNS.  |  Hashimotodani, Y., et al. 2007. Neuroscientist. 13: 127-37. PMID: 17404373
7. A duplication at chromosome 11q12.2-11q12.3 is associated with spinocerebellar ataxia type 20.  |  Knight, MA., et al. 2008. Hum Mol Genet. 17: 3847-53. PMID: 18801880
8. DAGLα Inhibition as a Non-invasive and Translational Model of Episodic Headache.  |  Levine, A., et al. 2020. Front Pharmacol. 11: 615028. PMID: 33584293
9. A fluorescence recovery after photobleaching protocol to measure surface diffusion of DAGLα in primary cultured cortical mouse neurons.  |  Yoon, S. and Penzes, P. 2022. STAR Protoc. 3: 101118. PMID: 35098165
10. Nutrient composition of different energy-restricted diets determines plasma endocannabinoid profiles and adipose tissue DAGL-α expression; a 12-week randomized controlled trial in subjects with abdominal obesity.  |  Wang, Y., et al. 2024. J Nutr Biochem. 128: 109605. PMID: 38401691
11. Deletion of endocannabinoid synthesizing enzyme DAGLα from cerebellar Purkinje cells decreases social preference and elevates anxiety.  |  Smith, G., et al. 2024. bioRxiv.. PMID: 39211155
12. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.  |  Ishikawa, K., et al. 1998. DNA Res. 5: 169-76. PMID: 9734811

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DAGLα Antibody (E-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-DAGLα Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DAGLα Antibody (E-6) Specifications

Alternate Product Name: DAGLα

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 887-1042

Epitope Target: DAGLα

Epitope Species: human

Product Reactivity/Detection: DAGLα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for DAGLα Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin