DACH2 Antibody (A-5): sc-515091
- DACH2 Antibody (A-5) is a mouse monoclonal IgG1 κ DACH2 antibody provided at 200 µg/ml
- raised against amino acids 227-315 mapping within an internal region of DACH2 of human origin
- DACH2 Antibody (A-5) is recommended for detection of DACH2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- Anti-DACH2 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-515091 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DACH2 Antibody (A-5) for WB applications. These reagents are now offered in bundles with DACH2 Antibody (A-5) (see ordering information below).
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DACH2 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects DACH2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DACH2 Antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DACH2, or Dachshund homolog 2, is a crucial transcription factor that plays a significant role in organogenesis and cell fate determination, particularly in the development of the eye, limb, and genital structures. This 599 amino acid protein is characterized by two distinct dachshund domains: an N-terminal domain that facilitates DNA binding and a C-terminal domain that mediates protein-protein interactions. DACH2 localization in the nucleus is vital for function as DACH2 regulates gene expression by interacting with other proteins, such as Six1 and EYA2, to promote myogenesis, while also repressing Six6 activity through direct inhibition of cyclin-dependent kinase inhibitors. Notably, DACH2 undergoes alternative splicing, resulting in three named isoforms, which may have distinct regulatory roles in various biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DACH2 Antibody (A-5) References:
- Synergistic regulation of vertebrate muscle development by Dach2, Eya2, and Six1, homologs of genes required for Drosophila eye formation. | Heanue, TA., et al. 1999. Genes Dev. 13: 3231-43. PMID: 10617572
- Characterization of mouse Dach2, a homologue of Drosophila dachshund. | Davis, RJ., et al. 2001. Mech Dev. 102: 169-79. PMID: 11287190
- Activation of myogenesis by the homeobox gene Lbx1 requires cell proliferation. | Mennerich, D. and Braun, T. 2001. EMBO J. 20: 7174-83. PMID: 11742994
- Pax3 and Dach2 positive regulation in the developing somite. | Kardon, G., et al. 2002. Dev Dyn. 224: 350-5. PMID: 12112464
- Targeted disruption of mouse Dach1 results in postnatal lethality. | Backman, M., et al. 2003. Dev Dyn. 226: 139-44. PMID: 12508235
- DACH1 inhibits transforming growth factor-beta signaling through binding Smad4. | Wu, K., et al. 2003. J Biol Chem. 278: 51673-84. PMID: 14525983
- Six1 controls patterning of the mouse otic vesicle. | Ozaki, H., et al. 2004. Development. 131: 551-62. PMID: 14695375
- Mouse Dach2 mutants do not exhibit gross defects in eye development or brain function. | Davis, RJ., et al. 2006. Genesis. 44: 84-92. PMID: 16470613
- Activity-dependent gene regulation in skeletal muscle is mediated by a histone deacetylase (HDAC)-Dach2-myogenin signal transduction cascade. | Tang, H. and Goldman, D. 2006. Proc Natl Acad Sci U S A. 103: 16977-82. PMID: 17075071
- Mouse Dach1 and Dach2 are redundantly required for Müllerian duct development. | Davis, RJ., et al. 2008. Genesis. 46: 205-13. PMID: 18395837
- Prospective validation of DACH2 as a novel biomarker for prediction of metastasis and prognosis in muscle-invasive urothelial carcinoma of the bladder. | He, YD., et al. 2015. Biochem Biophys Res Commun. 459: 416-23. PMID: 25744029
- Dach2-Hdac9 signaling regulates reinnervation of muscle endplates. | Macpherson, PC., et al. 2015. Development. 142: 4038-48. PMID: 26483211
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DACH2 Antibody (A-5) | sc-515091 | 200 µg/ml | $322.00 | |||
DACH2 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-531285 | 200 µg Ab; 10 µg BP | $361.00 | |||
DACH2 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-524932 | 200 µg Ab, 40 µg BP | $361.00 | |||
DACH2 Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-544467 | 200 µg Ab; 20 µg BP | $361.00 | |||
DACH2 Antibody (A-5) X | sc-515091 X | 200 µg/0.1 ml | $322.00 | |||
DACH2 Antibody (A-5) AC | sc-515091 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DACH2 Antibody (A-5) HRP | sc-515091 HRP | 200 µg/ml | $322.00 | |||
DACH2 Antibody (A-5) FITC | sc-515091 FITC | 200 µg/ml | $336.00 | |||
DACH2 Antibody (A-5) PE | sc-515091 PE | 200 µg/ml | $349.00 | |||
DACH2 Antibody (A-5) Alexa Fluor® 488 | sc-515091 AF488 | 200 µg/ml | $364.00 | |||
DACH2 Antibody (A-5) Alexa Fluor® 546 | sc-515091 AF546 | 200 µg/ml | $364.00 | |||
DACH2 Antibody (A-5) Alexa Fluor® 594 | sc-515091 AF594 | 200 µg/ml | $364.00 | |||
DACH2 Antibody (A-5) Alexa Fluor® 647 | sc-515091 AF647 | 200 µg/ml | $364.00 | |||
DACH2 Antibody (A-5) Alexa Fluor® 680 | sc-515091 AF680 | 200 µg/ml | $364.00 | |||
DACH2 Antibody (A-5) Alexa Fluor® 790 | sc-515091 AF790 | 200 µg/ml | $364.00 |