Dab2 Antibody (E-11): sc-136964

Dab2 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dab2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Dab2 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dab2 protein, also known as DOC-2, plays a crucial role as a mitogen-responsive phosphoprotein that interacts with the SH3 domain of GRB2, suggesting involvement in cellular signaling pathways that regulate growth and development. Dab2 protein is primarily located in the cytoplasm, where function as a negative regulator of growth is particularly important in cellular responses to mitogenic signals. Dab2′s ability to modulate growth signals is vital for maintaining cellular homeostasis and preventing uncontrolled cell proliferation, which can lead to tumorigenesis. Dab2′s interactions with other signaling proteins underscore significance in various cellular processes, including differentiation and migration, making Dab2 protein a valuable target for research in cancer biology and developmental studies.

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Dab2 Antibody (E-11) References:

1. DOC-2/DAB2 is the binding partner of myosin VI.  |  Inoue, A., et al. 2002. Biochem Biophys Res Commun. 292: 300-7. PMID: 11906161
2. Decreased DOC-2/DAB2 expression in urothelial carcinoma of the bladder.  |  Karam, JA., et al. 2007. Clin Cancer Res. 13: 4400-6. PMID: 17671122
3. Dab2 regulates clathrin assembly and cell spreading.  |  Chetrit, D., et al. 2009. Biochem J. 418: 701-15. PMID: 19000037
4. Negative regulation of the endocytic adaptor disabled-2 (Dab2) in mitosis.  |  Chetrit, D., et al. 2011. J Biol Chem. 286: 5392-403. PMID: 21097498
5. Disabled-2 (Dab2) inhibits Wnt/β-catenin signalling by binding LRP6 and promoting its internalization through clathrin.  |  Jiang, Y., et al. 2012. EMBO J. 31: 2336-49. PMID: 22491013
6. miR-93-directed downregulation of DAB2 defines a novel oncogenic pathway in lung cancer.  |  Du, L., et al. 2014. Oncogene. 33: 4307-15. PMID: 24037530
7. Dynamic multimerization of Dab2-Myosin VI complexes regulates cargo processivity while minimizing cortical actin reorganization.  |  Rai, A., et al. 2021. J Biol Chem. 296: 100232. PMID: 33372034
8. The reeler gene-associated antigen on Cajal-Retzius neurons is a crucial molecule for laminar organization of cortical neurons.  |  Ogawa, M., et al. 1995. Neuron. 14: 899-912. PMID: 7748558
9. Mouse disabled (mDab1): a Src binding protein implicated in neuronal development.  |  Howell, BW., et al. 1997. EMBO J. 16: 121-32. PMID: 9009273
10. Neuronal position in the developing brain is regulated by mouse disabled-1.  |  Howell, BW., et al. 1997. Nature. 389: 733-7. PMID: 9338785
11. Disabled-2 (Dab2) is an SH3 domain-binding partner of Grb2.  |  Xu, XX., et al. 1998. Oncogene. 16: 1561-9. PMID: 9569023
12. Disabled-1 acts downstream of Reelin in a signaling pathway that controls laminar organization in the mammalian brain.  |  Rice, DS., et al. 1998. Development. 125: 3719-29. PMID: 9716537

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dab2 Antibody (E-11) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Dab2 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dab2 Antibody (E-11) Specifications

Alternate Product Name: DAB2 Clathrin Adaptor Protein

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 661-770

Epitope Target: Dab2

Epitope Species: human

Product Reactivity/Detection: Dab2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Dab2 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin