DAAO Antibody (B-3): sc-398757
- DAAO Antibody (B-3) is a mouse monoclonal IgG2a κ DAAO antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 312-337 near the C-terminus of DAAO of mouse origin
- DAAO Antibody (B-3) is recommended for detection of DAAO of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DAAO Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DAAO Antibody (B-3) for WB and IHC(P) applications. These reagents are now offered in bundles with DAAO Antibody (B-3) (see ordering information below).
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DAAO Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects DAAO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DAAO Antibody (B-3) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. D-amino acid oxidase (DAAO) plays a crucial role in metabolizing exogenous D-amino acids, which can accumulate during aging, and is essential for modulating D-serine levels in the brain, acting as a detoxifying agent. DAAO specifically targets D-configured amino acids and shows preference for those with small, hydrophobic side chains, as well as polar, aromatic, and basic functional groups. Single base-pair substitutions at specific amino acid residues can lead to DAAO activity loss in mice, highlighting DAAO′s sensitivity to genetic variations. A mutation at residue Y238, a conserved residue in the active site, significantly slows product release compared to wild-type enzyme, underscoring Y238′s importance in regulating active site access. While other conserved residues in the active site don′t participate in acid-base catalysis, these residues remain critical for substrate interactions, suggesting a hybrid transfer mechanism for catalysis influenced by substrate orientation within the active site.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DAAO Antibody (B-3) References:
- D-Amino acid oxidase: new findings. | Pilone, MS. 2000. Cell Mol Life Sci. 57: 1732-47. PMID: 11130179
- D-amino acid oxidase: its potential in the production of 7-aminocephalosporanic acid. | Mujawar, SK. 1999. Hindustan Antibiot Bull. 41: 1-14. PMID: 12024974
- Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study. | Boselli, A., et al. 2002. Eur J Biochem. 269: 4762-71. PMID: 12354107
- A single-base-pair substitution abolishes D-amino-acid oxidase activity in the mouse. | Sasaki, M., et al. 1992. Biochim Biophys Acta. 1139: 315-8. PMID: 1355365
- Competitive Inhibitors Unveil Structure/Function Relationships in Human D-Amino Acid Oxidase. | Molla, G. 2017. Front Mol Biosci. 4: 80. PMID: 29250527
- Biochemical Properties of Human D-Amino Acid Oxidase. | Murtas, G., et al. 2017. Front Mol Biosci. 4: 88. PMID: 29326945
- D-Amino Acid Oxidase-pLG72 Interaction and D-Serine Modulation. | Pollegioni, L., et al. 2018. Front Mol Biosci. 5: 3. PMID: 29417050
- Dual role of D-amino acid oxidase in experimental pain models. | Sharma, J., et al. 2019. Eur J Pharmacol. 855: 98-102. PMID: 31059710
- Overexpression of D-amino acid oxidase prevents retinal neurovascular pathologies in diabetic rats. | Jiang, H., et al. 2021. Diabetologia. 64: 693-706. PMID: 33319325
- Antimicrobial D-amino acid oxidase-derived peptides specify gut microbiota. | Murtas, G., et al. 2021. Cell Mol Life Sci. 78: 3607-3620. PMID: 33484270
- Astrocytic d-amino acid oxidase degrades d-serine in the hindbrain. | Gonda, Y., et al. 2022. FEBS Lett. 596: 2889-2897. PMID: 35665501
- Targeting D-Amino Acid Oxidase (DAAO) for the Treatment of Schizophrenia: Rationale and Current Status of Research. | Kuo, CY., et al. 2022. CNS Drugs. 36: 1143-1153. PMID: 36194364
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAAO Antibody (B-3) | sc-398757 | 200 µg/ml | $322.00 | |||
DAAO Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-530919 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAAO Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-524473 | 200 µg Ab, 40 µg BP | $361.00 | |||
DAAO Antibody (B-3): m-IgG2a BP-HRP Bundle | sc-547631 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAAO Antibody (B-3) AC | sc-398757 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DAAO Antibody (B-3) HRP | sc-398757 HRP | 200 µg/ml | $322.00 | |||
DAAO Antibody (B-3) FITC | sc-398757 FITC | 200 µg/ml | $336.00 | |||
DAAO Antibody (B-3) PE | sc-398757 PE | 200 µg/ml | $349.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 488 | sc-398757 AF488 | 200 µg/ml | $364.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 546 | sc-398757 AF546 | 200 µg/ml | $364.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 594 | sc-398757 AF594 | 200 µg/ml | $364.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 647 | sc-398757 AF647 | 200 µg/ml | $364.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 680 | sc-398757 AF680 | 200 µg/ml | $364.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 790 | sc-398757 AF790 | 200 µg/ml | $364.00 | |||
DAAO (B-3) Neutralizing Peptide | sc-398757 P | 100 µg/0.5 ml | $69.00 |